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园艺学报 ›› 2019, Vol. 46 ›› Issue (2): 205-214.doi: 10.16420/j.issn.0513-353x.2018-0405

• 研究论文 •    下一篇

葡萄叶片衰老过程中不同光质对其光合和叶绿体超微结构的影响

王海波,王 帅,王孝娣,史祥宾,王志强,刘凤之*   

  1. 中国农业科学院果树研究所,农业部园艺作物种质资源利用重点实验室,辽宁省落叶果树矿质营养与肥料高效利用重点实验室,辽宁兴城 125100
  • 出版日期:2019-02-25 发布日期:2019-02-25
  • 基金资助:

    中国农业科学院创新工程项目(CAAS-ASTIP-2017-RIP-04);国家现代农业产业技术体系建设专项资金项目(CARS-29-zp-4);农业部“948”重点项目(2011-G28)

Effect of Different Light Qualities on the Photosynthetic Properties and Ultrastructure of Chloroplast in Senescing Grape Leaves

WANG Haibo,WANG Shuai,WANG Xiaodi,SHI Xiangbin,WANG Zhiqiang,and LIU Fengzhi*   

  1. Fruit Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Germplasm Resources Utilization of Horticultural Crops,Ministry of Agriculture,Key Laboratory of Mineral Nutrition and Fertilizers Efficient Utilization of Deciduous Fruit Tree,Liaoning Province,Xingcheng,Liaoning 125100,China
  • Online:2019-02-25 Published:2019-02-25

摘要:

以栽植于日光温室中适宜延迟栽培的‘贝达’砧‘意大利’葡萄为试材,自2013年8月1日始至落叶(2014年1月31日)止每天分别进行红光和蓝光不同光质延长光照至24:00补光处理,以不补光作为对照,研究不同光质补光处理对葡萄叶片衰老过程中叶片的外观形态特征、叶绿素含量、净光合速率、Fv/Fm、可溶性蛋白质含量和叶绿体超微结构的影响,为叶片抗衰老技术的研发提供理论依据。研究结果表明:补充红光显著提高了叶片的叶绿素含量、净光合速率、Fv/Fm值和可溶性蛋白质含量,叶绿体基粒片层排列整齐有序,明显减缓了叶绿体超微结构的解体,显著延缓叶片衰老;补充蓝光处理前期显著降低了叶片的叶绿素含量、净光合速率、Fv/Fm值和可溶性蛋白质含量,破坏了叶绿体的超微结构,加速了植株的衰老进程;但在叶片衰老后期,叶绿素含量、净光合速率、Fv/Fm值和可溶性蛋白质含量逐渐高于对照,并且叶绿体的基粒片层比对照排列整齐有序,在一定程度上延缓了叶片衰老。补充蓝光处理的叶片可溶性蛋白质含量一直显著高于补充红光和对照。综上,补充红光处理有效延缓了叶片衰老进程,延长了叶片的生理功能期。

关键词: 葡萄, 叶片, 衰老, 补充光照, 红光, 蓝光, 光合特性, 叶绿体超微结构

Abstract:

The experiment was carried out in 2013 at the fruit garden of the Chinese Academy of Agricultural Sciences in Liaoning,which was equipped with a core grape technology demonstration garden.‘Italy’grape cultivars were grafted on Beta as test materials. And their row spacing was 0.7 m,line spacing was 2 m. The lean dragon trunk matches the V shape leaf curtain,and used the integrated management of water and fertilizer,other management was the same as the routine. Since the beginning of August 1st to the defoliation day before dark for half an hour to fill 24:00,use red light and blue light to deal with the grape cultivars,with no-fill-light grapes as contrast,studying morphological characteristics,chlorophyll content,net photosynthetic rate,Fv/Fm value,soluble proteins content and chloroplast ultrastructure of grape leaves in aging process,to provide theoretical basis for the research and development of leaf anti-aging technology. The research result showed that compared with the control group,red light treatment increased the chlorophyll content,net photosynthetic rate,Fv/Fm value and soluble proteins content of leaf,the chloroplast grana were arranged in order,the disintegration of chloroplast ultrastructure was obviously slowed down,leaf senescence was delayed significantly. Blue light treatment significantly reduced the leaf chlorophyll content,net photosynthetic rate,soluble proteins content and Fv/Fm value,the ultrastructure of chloroplasts was destroyed,and the aging process of grapes was accelerated. But in the later stage of leaf senescence,the chlorophyll content of leaf,the net photosynthetic rate,soluble proteins content and Fv/Fm value of the blue light treatment was higher than that of the control. Moreover,the grana lamellae of leaf chloroplasts are arranged in order,leaf senescence was delayed in some degree. The content of soluble proteins in leaf of blue light treatment was significantly higher than that of red light treatment and control group. Summing up the above,the red light as supplement light had the best effect,the leaf senescence process was delayed effectively,and the physiological function of leaves was prolonged.

Key words: grape, leaf, senescence, fill light, red light, blue light, photosynthetic property, chloroplast ultrastructure

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