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园艺学报 ›› 2017, Vol. 44 ›› Issue (8): 1429-1438.doi: 10.16420/j.issn.0513-353x.2016-0905

• 研究论文 •    下一篇

桃PpSnRK1β1和PpSnRK1β2在番茄中超表达对光合碳代谢的影响

王贵芳1,彭福田2,*,于 雯2,赵永飞2,罗静静2,肖元松2,陈晓璐2   

  1. (1山东省果树研究所,山东泰安 271000;2山东农业大学园艺科学与工程学院,山东泰安 271018)
  • 出版日期:2017-08-25 发布日期:2017-08-25

Effects of Over-expression of Peach PpSnRK1β1 and PpSnRK1β2 on Photosynthetic Carbohydrate Metabolism in Tomato

WANG Guifang1,PENG Futian2,*,YU Wen2,ZHAO Yongfei2,LUO Jingjing2,XIAO Yuansong2,and CHEN Xiaolu2   

  1. (1Shandong Institute of Pomology,Tai’an,Shandong 271000,China;2 College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China)
  • Online:2017-08-25 Published:2017-08-25

摘要:

从毛桃[Prunus persicaLinn.Batsch.]叶片中克隆了PpSnRK1蛋白激酶β亚基编码基因PpSnRK1β1PpSnRK1β2,其cDNA全长分别为720 bp867 bpqRT-PCR组织表达分析表明,PpSnRK1β1在‘鲁星’桃叶片中的表达量最高,PpSnRK1β2在其果实中表达量最高,两者在其花中的表达量均较低。通过农杆菌介导转化番茄,获得超表达PpSnRK1β1的番茄株系T21T23和超表达PpSnRK1β2的株系T91T92。研究发现,与野生型(WT)相比,超表达株系T23T91叶片的SnRK1蛋白激酶活性分别增加9.84%53.32%T91叶片净光合速率比WT提高17.02%;叶片可溶性糖含量比WT增加34.00%T91T92叶片淀粉含量分别约是WT2倍。T23T91T92果实维生素C含量分别比WT下降19.21%38.49%39.76%。因此说明PpSnRK1β1PpSnRK1β2参与了光合作用过程,调控碳代谢及次生代谢过程

关键词: 桃, PpSnRK1β1, PpSnRK1β2, 超表达, 番茄, 光合作用, 碳代谢

Abstract: PpSnRK1 kinase β subunit encoding gene(PpSnRK1β1 and PpSnRK1β2)were primarily cloned from peach [Prunus persica(Linn.)Batsch.] leaves. The CDS of PpSnRK1β1 and PpSnRK1β2 are 720 bp and 867 bp respectively. Real-time quantitative polymerase chain reaction(qRT-PCR)analysis revealed that PpSnRK1β1 expressed the highest in leaves of‘Luxing’peach,while PpSnRK1β2 expressed the highest in it’s fruits,both expressed the lowest in it’s flowers. By Agrobacterium mediated transformation,over-expression of transgenic PpSnRK1β1(line T21 and T23)and PpSnRK1β2(line T91 and T92)tomato plants were obtained. Compared with wild type tomato(WT)activities of SnRK1 in young leaves of line T23 and T91 were increased by 9.84% and 53.32%,respectively. The photosynthetic rate in leaves of T91 was 17.02% higher than that of WT. The soluble sugar content in leaves was increased by 34.00% compared with WT. The starch content in leaves of T91 and T92 was nearly doubled compared with that in WT. Instead,the content of vitamin C in fruits of T23,T91 and T92 decreased by 19.21%,38.49% and 39.76% compared with WT. These data demonstrate that PpSnRK1β1 and PpSnRK1β2 are involved in photosynthesis,and are regulated in carbohydrate metabolism and secondary metabolism.

Key words: Prunus persica, PpSnRK1β1, PpSnRK1β2, over-expression, tomato, photosynthesis, carbohydrate metabolism

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