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园艺学报 ›› 2015, Vol. 42 ›› Issue (7): 1356-1366.doi: 10.16420/j.issn.0513-353x.2014-1000

• 观赏植物 • 上一篇    下一篇

乙烯和葡萄糖处理对‘洛阳红’牡丹切花花色和花青素苷合成的影响

高树林,张超,杜丹妮,刘爱青,董丽   

  1. 1北京林业大学园林学院,花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,北京 100083;2曹州牡丹花木开发总公司,山东菏泽 274000
  • 出版日期:2015-07-25 发布日期:2015-07-25
  • 基金资助:
    高等学校博士学科点专项科研基金项目(20130014110014)

Effect of Glucose and Ethylene on Flower Color and Anthocyanin Biosynthesis in Tree Peony‘Luoyanghong’Cut Flower

GAO Shu-Lin, ZHANG Chao, DU Dan-Ni, LIU Ai-Qing, DONG Li   

  1. 1College of Landscape Architecture,Beijing Forestry University,Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding,National Flower Engineering Research Center for Floriculture,Beijing 100083,China;2Caozhou Gen Devt Comp of Peony Flower,Heze,Shandong 274000,China
  • Online:2015-07-25 Published:2015-07-25

摘要: 以‘洛阳红’牡丹(Paeonia suffruticosa‘Luoyanghong’)切花为试材,研究10 μL ? L-1乙烯处理、1 μL ? L-1乙烯抑制剂1-MCP处理、90 g ? L-1葡萄糖处理、90 g ? L-1葡萄糖 + 10 μL ? L-1乙烯复合处理对切花花色和花青素苷含量的影响,以蒸馏水处理为对照。研究结果表明:乙烯处理的切花花色明度增加,红度和彩度下降,花瓣花青素苷含量下降;1-MCP处理与对照无显著差异;葡萄糖处理花色明度下降,红度和彩度增加,花瓣花青素苷含量增加;葡萄糖乙烯复合处理对切花花色及花青素苷积累也有明显改善与促进作用。对花青素苷合成相关基因表达量分析的结果表明,乙烯处理对基因表达有抑制作用,而葡萄糖处理有显著的正调控作用,葡萄糖乙烯复合处理则显著缓解了单独乙烯处理对基因表达的负调控作用。葡萄糖信号和乙烯信号之间一定程度上存在互作,葡萄糖缓解了乙烯对牡丹切花花青素苷合成的抑制作用。

关键词: 牡丹, 切花, 乙烯, 葡萄糖, 花色, 花青素苷

Abstract: The effects of different treatments(10 μL ? L-1 ethylene,1 μL ? L-1 ethylene inhibitor 1-MCP,90 g ? L-1 glucose and 90 g ? L-1 glucose in combination with 10 μL ? L-1 ethylene)on flower color and anthocyanin biosynthesis in the cut flowers of a tree peony(Paeonia suffruticosa)purplish red-?owered cultivar‘Luoyanghong’were investigated. Results showed that flowers under ethylene treatment presented higher lightness,lower redness and chroma,as well as decreased anthocyanin content compared to ones from the control treatment. However,1-MCP treatment had no significant effects on the cut flowers. Glucose treatment prominently decreased lightness,increased redness and chroma of theflowers,and enhanced the anthocyanin accumulation. Similarly,glucose-ethylene combined treatment also marked beneficial effects on the coloration and anthocyanin accumulation. qRT-PCR was used to analyze the relative expression levels of anthocyanin biosynthetic genes and results showed that ethylene treatment significantly inhibited the expression of all tested genes,while glucose enhanced the expression of most genes. Glucose-ethylene combined treatment significantly up-regulated the genes,which would be inhibited by ethylene solo treatment. According to all above results,it can be concluded that petal coloration and anthocyanin biosynthesis in tree peony‘Luoyang Hong’cut flower is negatively regulated by ethylene signaling but positively regulated by glucose signaling. Moreover,ethylene signaling and glucose signaling have an antagonism effect on anthocyanin biosynthesis,since glucose increased anthocyanin accumulation which was inhibited by ethylene.

Key words: Paeonia suffruticosa, cut flower, ethylene, glucose, flower color, anthocyanin

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