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ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (3): 437-440.

• 研究简报 • Previous Articles     Next Articles

Preliminary identification of Tospovirus on tomato in Jianshui county of Yunnan province

LIU Ya-ting1,3,ZHENG Yuan-xian1,LI Yong-zhong2,LI Zheng-yue3*   

  1. (1College of Agricultural and Biotechnology, Yunnan Agricultural University,Yunnan 650201, China;2College of Tobacco Science, Yunnan Agricultural University,Yunnan 650201, China;3College of plant protection, Yunnan Agricultural University,Yunnan 650201, China)
  • Received:2008-10-09 Revised:2009-02-04 Online:2009-03-25 Published:2009-03-25
  • Contact: LI Zheng-yue

Abstract:

Nine samples possibly from genus Tospovirus were collected from major vegetable-growing field in Yunnan province, including Honghe, Chuxiong and Kunming, during 2005 - 2006 period. Around 500 bp fragments were obtained after RT-PCR were executed on 9 samples using Tospovirus Group Primers. The sequence results of around 500 bp fragments were compared in the NCBI BLAST website, one sample from tomato in Honghe county of Yuannan province was identified as Tospovirus genus. A phylogenetic tree was constructed using DNAMAN software according to the sequence of the sample and their corresponding public RNA L fragment sequences of Tospovirus. The results indicated that this sample obtained in this study fell into the Serogroup Ⅳ.

Key words: Tospovirus, Tomato, Serogroup

CLC Number: