Clon ing of Two Different ent-kaurene oxidase (STGA3) Sequence in Strawberry

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 128-129.

• 研究简报 • Previous Articles Next Articles

Clon ing of Two Different ent-kaurene oxidase (STGA3) Sequence in Strawberry

Shi Yanjing¹ ;Sun Zhongxu²;Shu Huairui²

(¹Chemistry Engineering College, Qingdao University of Science and Technology, Qingdao 266042, China; ² Institute of Horticulture Science and Engineering, Shandong Agriculture University, Tai'an 271018, China )

Received:2005-04-11 Revised:2005-07-29 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: A pair of degenerate p rimers were designed according to the conservative amino acid sequences to clone the ent-kaurene oxidase from strawberry, and obtained two different RT-PCR p roducts from the strawberry leaves and flowers respectively. These two segments have different nucleotide equences, and Southern blot detected 3 cop ies of target gene in the strawberry genome. So there are at least two different genes coding ent-kaurene oxidase to respond different development signals.

Key words: straberry, gibberellins, GA3 gene, Ent-kaurene oxidase, Biosynthesis

Shi Yanjing;Sun Zhongxu;Shu Huairui. Clon ing of Two Different ent-kaurene oxidase (STGA3) Sequence in Strawberry[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 128-129.

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Clon ing of Two Different ent-kaurene oxidase (STGA3) Sequence in Strawberry

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