Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 985-990.

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Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

ZHENG Ya-dong¹^*； GUO Yu-long¹^*； CHEN Xu²； LI Yan-dong¹； OU Jian-long³； and LI Ming-yang^1**

(¹College of Horticulture and Landscape, Southwest University, Chongqing 400715, China; ²Chongqing Center of Bio-engineering for Crops, Chongqing 400055, China; ³Chongqing Academy of Agricultural Technology Promotion, Chongqing 400020, China)

Received:2007-02-13 Revised:2007-05-28 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract:

An AGAMOUS homologue (GhMADS3) was introduced into petunia via Agrobacterium tume

faciensmediated method. Forteen independent kanamycin-resistant petunia plants were alive. GUS staining revealed that ectopic gene was expressed in transgenic plants. PCR detection showed that the GhMADS3 was integrated into their genomes. The expression of the GhMADS3 was detected by RT-PCR. Constitutive expression

of GhMADS3 resulted in visible phenotypic alterations in floral organ. In transgenic petunia plants, GhMADS3

resulted in the sepals swelling, corolla smaller and corolla deep spliting. It was noteworthy that the color of

transgenic floral organ became weaker or entirely pale. The measuration of anthocyanin and chlorophyll showed

that the content in transgenic plants were greatly lower than wild-type. It also indicated that GhMADS3 in

plants has some relationship with the formation of flower color.

Key words: Petunia, Flower color, Flower shape, MADS-box, GhMADS3

ZHENG Ya-dong;GUO Yu-long;CHEN Xu;LI Yan-dong;OU Jian-long;and LI Ming-yang.

Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 985-990.

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Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

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