Abstract: Based on the information of the established core collection，genetic diversity of 615 typical accessions representing 50 years of breeding program selected from a collection of 3 026 processing tomato inbred lines was analyzed and compared by using 16 phenotypes and 81 polymorphic markers（13 SSR，24 InDel and 44 SNP）. The results indicate that all inbred lines were divided into two groups by either phenotypes or genotypes，and show the consistent results by each other. Furthermore these two groups could be classified into 7 and 6 sub-groups respectively. When compared to the categories respectively obtained from both phenotypes and genotypes，we found that most of the early released inbred lines were clustered in groupⅠwhile the developed modern lines were involved randomly in both groupⅠandⅡ. It hints that the newly inbred lines showed much more diverse than the old population. In addition，SNP markers show more polymorphic than SSR and InDel markers as indicated by allele number，gene diversity and polymorphism information content.

Key words: processing tomato, molecular marker, polymorphism, genetic variation