RT-PCR Detection and Molecular Variability of Apple stem pitting virus

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (1): 9-16.

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RT-PCR Detection and Molecular Variability of Apple stem pitting virus

LI Li-li1;DONG Ya-feng2*;ZHANG Zun-ping2;ZHANG Zhi-hong1;FAN Xu-dong2;PEI Guang-qian2

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; ²Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China )

Received:2009-09-07 Revised:2009-11-11 Online:2010-01-25 Published:2010-01-25
Contact: DONG Ya-feng

Abstract

Abstract: Thirty-three samples including 6 apple and 7 pear cultivars were detected for Apple stem pitting virus (ASPV) by RT-PCR, the infected rate was up to 63% (19 /33). The ASPV coat protein (CP) gene of 6 Chinese isolates were amplified by RT-PCR, cloned and sequenced. Sequence analysis showed that the full-length CP gene comprised 1 191 nt and encoded 397 amino acid. Identities of nucleotide between Chinese isolates and other isolates were 70.2% - 91.7%. Phylogenetic analysis showed that all isolates of ASPV fell into three groups, and the group swere slightly related to host, but it was not related to geographical origin of isolates. In group 1 and 3, the host of all isolates were apples. Six isolates came from pears and two isolates came from apples lay in group 2. The diversity of antigenic index was complicated for different ASPV isolates, and we presumed that the diversity of antigenic index was due to variation of sequences, so serology differentiations were likely to be gradually developed.

Key words: apple, pear, Apple stem pitting virus, RT-PCR detection, sequence analysis

CLC Number:

S661
S718.85

LI Li-li;DONG Ya-feng;ZHANG Zun-ping;ZHANG Zhi-hong;FAN Xu-dong;PEI Guang-qian. RT-PCR Detection and Molecular Variability of Apple stem pitting virus[J]. ACTA HORTICULTURAE SINICA, 2010, 37(1): 9-16.

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RT-PCR Detection and Molecular Variability of Apple stem pitting virus

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