In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSVby RT2PCR

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 87-89.

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In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSVby RT2PCR

Dai Hongyan¹;Zhang Zhihong¹;Wu Luping¹;Hou Yilong^1,2;LüDeguo¹

(¹College of Horticulture , Shenyang Agricultural University , Shenyang 110161 , China ;²College of Biotechnology , Dalian University,Dalian 116622 , China)

Received:2002-03-12 Revised:2002-07-23 Online:2003-02-25 Published:2003-02-25

Abstract

Abstract: The effects of size of inoculated shoot tip , method of inoculation , medium and genotype on shoot tip culture of sweet cherry ( Prunus avium L. ) cultivars were studied. The rate of shoots forming from tips of one-year-old twigs was low , 8. 3 % to 23. 7 % , while the rate of shoots forming from tips of actively growing shoots was higher , 27. 3 % to 37. 5 %. Prunus necrotic ringspot virus (PNRSV) was detected early in some of sweet cherry plantlets in vitro with RT-PCR technique. Plants free of PNRSV were found. It was proved that PNRSV could not be eliminated effectively from sweet cherry plantlets in vitro by culture of minor shoot tip.

Key words: Sweet cherry, Shoot tip culture, Virus, RT-PCR

CLC Number:

S 662. 5

Dai Hongyan;Zhang Zhihong;Wu Luping;Hou Yilong;LüDeguo. In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSVby RT2PCR[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 87-89.

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In Vitro Shoot Tip Culture of Sweet Cherry Cultivars and Detection of PNRSVby RT2PCR

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