Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1136-1144.doi: 10.16420/j.issn.0513-353x.2021-0221

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Molecular Identification and Sequence Analysis of Begomoviruses Infecting Impatiens balsamina

ZHONG Jing, ZHAO Liling, LI Tingting, ZHANG Shuiying, CHEN Yue, DING Ming()   

  1. Key Laboratory of Agricultural Biotechnology of Yunnan Province,Key Laboratory of the Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs,Institute of Biotechnology and Germplasm Resources,Yunnan Academy of Agricultural Sciences,Kunming 650223,China
  • Received:2021-11-25 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
  • Contact: DING Ming


An investigation about diseases in Xishuangbanna Autonomous Prefecture,Yunnan Province identified an Impatiens balsamina plant with leaf shrinking and mosaic symptoms typical of begomovirus infections. To identify the possible virus associated with the disease,the sample was collected and detected by PCR with begomovirus degenerate primers. The amplicons were cloned and sequenced,and two sequences(YN6198-28 and YN6198-31)of 2 763 bp corresponding to begomovirus DNA-A genome with six open reading frames were identified from this sample. The YN6198-28 DNA-A sequence shares the highest identity(94.90%)with Ludwigia yellow vein Vietnam virus(LuYVVNV),which confirmed that the virus was an isolate of LuYVVNV. Recombination analysis revealed that the YN6198-28 isolate is a recombinant begomovirus derived from Ludwigia yellow vein virus and Ageratum yellow vein China virus.

Key words: Impatiens balsamina, Begomoviruses, recombination virus

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