Somatic Embryo Genesis from in Vitro Leaves of Pear

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (1): 85-86.

• 研究报告 • Previous Articles Next Articles

Somatic Embryo Genesis from in Vitro Leaves of Pear

Sun Qingrong¹;Liu Qingzhong¹;Zhao Ruihua²

(¹ Shandong Institute of Pomology , Tai’an 271000 , China ; ² College of Life Science , Shandong Agricultural University , Tai’an 271000 , China)

Received:2002-03-12 Revised:2002-07-23 Online:2003-02-25 Published:2003-02-25

Abstract

Abstract:

Young leaves in vitro of pear ( Pyrus communis L. ) cultivars Red D’Anjou and D’Anjou were selected as materials. Effect of media on shoot and somatic embryo regeneration from leaves was studied. Results showed that the composition of basic media was the main factor affecting shoot regeneration or somatic embryo regeneration from leaves ; the kind and concentration of exogenous hormone supplemented were important factor to higher
frequency regeneration of somatic embryo. In this study , somatic embryo regeneration rate was the highest in the treatment with medium NN69 supplemented with 2 mg/ L TDZ and 0. 1 mg/ L NAA , D’Anjou was 55. 5 % , and Red D’Anjou was 47. 3 %. Shoot regeneration rate was the highest on NN69 supplemented with 5 mg/ L BA and 0. 1mg/ L NAA , D’Anjou was 100 % , Red D’Anjou was 85. 7 %.

Key words: Pear ( Pyrus communis L. ), Leaves, In vitro, Somatic embryo, Regeneration

CLC Number:

S 661. 2

Sun Qingrong;Liu Qingzhong;Zhao Ruihua. Somatic Embryo Genesis from in Vitro Leaves of Pear[J]. ACTA HORTICULTURAE SINICA, 2003, 30(1): 85-86.

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Somatic Embryo Genesis from in Vitro Leaves of Pear

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