Cultivated Substrates Evaluation for in-situ Observation of Potato Tubers Using Computed Tomography

doi:10.16420/j.issn.0513-353x.2022-0218

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (5): 1085-1094.doi: 10.16420/j.issn.0513-353x.2022-0218

• Cultivation Physiology & Biochemistry • Previous Articles Next Articles

Cultivated Substrates Evaluation for in-situ Observation of Potato Tubers Using Computed Tomography

TAN Jie, LIU Jiangang, LIU Ju, JIAN Yinqiao, LI Guangcun, JIN Liping, XU Jianfei()

Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crops，Ministry of Agriculture Rural Affairs，State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2022-11-03 Revised:2023-01-16 Online:2023-05-25 Published:2023-05-31
Contact: XU Jianfei E-mail:xujianfei@caas.cn

Abstract

Abstract:

Soil hinders visualization in tuber growing and developing process，while Computed tomography（CT）technology can avoid destructive sampling and be applied on real-time observation for underground parts of plant. In order to select the cultivation substrate with good CT imaging of potato tubers，CT imaging effect，data extraction and plant growth parameters of six common cultivation substrates with different water content were compared and analyzed. The results showed that potato overground parts in sieved turf grew best，and furthermore，dry weight and fresh weight of tubers in sieved turf or vermiculite & sieved turf were the highest among all substrates. Except for vermiculite，the CT imaging can be improved with water content of substrates decreasing whereas potato plant can not grow normally under serious water shortage. In comparison to other substrates with same water content，there are less background impurities and better CT imaging sieved turf has. Therefore，sieved turf with 20% water content is a suitable cultivation substrate to observe tuber development using CT scanning.

Key words: potato, tuber, CT, cultivated substrate, growth and development

CLC Number:

S532

TAN Jie, LIU Jiangang, LIU Ju, JIAN Yinqiao, LI Guangcun, JIN Liping, XU Jianfei. Cultivated Substrates Evaluation for in-situ Observation of Potato Tubers Using Computed Tomography[J]. Acta Horticulturae Sinica, 2023, 50(5): 1085-1094.

Figures/Tables 9

Table 1 Default parameters of VPP

特征 Property	默认值 Default value
评估阈值 Evaluation threshold	0.04
用于评估的核大小 Kernel size for evaluation	1
核大小腐蚀 Kernel size erosion	5
核大小中值 Kernel size median	4
最小管衰减值 Minimal tube attenuation value	0.14
最大管衰减值 Maximal tube attenuation value	0.20
最小直径 Minimal diameter	50
最小球面比 Minimal spherical ratio	0.3

Table 2 Gray values of tubers and substrates in different substrates with 20% water content

基质类型 Substrate type	块茎灰度值 Gray values of tuber	基质灰度值 Gray values of substrate
ST	6 800 ~ 8 000	1 400 ~ 3 800
PL	6 800 ~ 8 400	1 000 ~ 4 500
PL & ST	6 700 ~ 7 600	1 700 ~ 4 500
GT	6 700 ~ 7 800	1 600 ~ 5 000
VM & ST	6 900 ~ 7 500	1 800 ~ 6 000
VM	7 200 ~ 8 300	4 300 ~ 9 800

Fig. 1 CT imaging in different substrates and empty basin with different water content ST：Sieved turf；PL：Perlite；PL & ST：Perlite mixed with sieved turf；GT：General turf；VM & ST：Vermiculite mixed with sievedturf；VM：Vermiculite. The same below.

Table 3 Comparison number of tubers between observed and measured with machine in different water content

基质类型 Substrate type	实测块茎数 Number of tubers observed	0含水量 0 water content		10%含水量10% water content		20%含水量20% water content
基质类型 Substrate type	实测块茎数 Number of tubers observed	机测块茎数 Number of tubers measured with machine	相对误差率/% Relative error rate	机测块茎数 Number of tubers measured with machine	相对误差率/% Relative error rate	机测块茎数 Number of tubers measured with machine	相对误差率/% Relative error rate
ST	10	10	0	10	0	7	-30.00
PL	18	17	-5.56	14	-22.22	12	-33.33
GT	20	19	-5.00	21	5.00	18	-10.00
VM	10	0	-100.00	1	-90.00	6	-40.00
PL & ST	13	12	-7.69	11	-15.38	11	-15.38
VM & ST	13	14	7.69	20	53.85	15	15.38

Fig. 2 Observed value and machine measured value of tuber volume in six substrates with different water content

Fig. 3 Growth parameters of aboveground plant in different substrate conditions Different lowercase letters indicate significant differences at 0.05 level. The same below.

Fig. 4 Dry weight and fresh weight of tubers in different substrate conditions

Fig. 5 Effect on CT imaging in different substrates with different water content The coordinate axis with the center of the flowerpot as the origin is composed of red horizontal lines，blue vertical lines and horizontal lines extending to the back of the flowerpot.

Fig. 6 CT observation of tuber development process in sieved turf A：Emergence stage；B：Stolon elongation stage；C：Stolon swelling stage；D：Tuber forming stage；E and F：Tuber bulking stage.

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Cultivated Substrates Evaluation for in-situ Observation of Potato Tubers Using Computed Tomography

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