ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (5): 864-876.doi: 10.16420/j.issn.0513-353x.2018-0924

• Research Papers • Previous Articles     Next Articles

Cloning and Functional Analysis of microRNA171 in Cucumber

ZHU Zaobing,YU Xiaqing*,ZHAI Yufei,WANG Panqiao,ZHAO Qinzheng,LI Ji,LOU Qunfeng,and CHEN Jinfeng*   

  1. College of Horticulture,Nanjing Agricultural University,National Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,China
  • Online:2019-05-25 Published:2019-05-25


To investigate the function of microRNA171(miR171)in cucumber,four precursors Csa-miR171a,Csa-miR171b,Csa-miR171c,Csa-miR171d of Csa-miR171 family genes were cloned in the northern Chinese cucumber‘Beijingjietou’and followed by sequence alignment and phylogenetic analysis. Target genes of the mature sequence of Csa-miR171 were predicted and functional analysis was performed in Arabidopsis by genetic transformation. The results showed that the two mature sequences,Csa-miR171a and Csa-miR171b were cleaved from the four Csa-miR171 precursors. Phylogenetic analysis showed that Csa-miR171a,Csa-miR171b,Csa-miR171c,Csa-miR171d were closer to Cme-miR171e,Cme-miR171c,Cme-miR171a,Cme-miR171b in melon than those in Arabidopsis,rice and tomato,etc. GRAS transcription factor family was predicted to be the main target gene of Csa-miR171. Overexpression of Csa-miR171a in Arabidopsis significantly affected the phenotypes including leaf morphology and leaf color,blooming time,root,fruit and floral morphology.

Key words: cucumber, microRNA171, clone, target gene prediction, transgenic

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