Karyotype and Fertility Analysis of Allotetraploids in Tree Peony（Paeonia section Moutan）

doi:10.16420/j.issn.0513-353x.2022-0413

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (7): 1444-1454.doi: 10.16420/j.issn.0513-353x.2022-0413

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Karyotype and Fertility Analysis of Allotetraploids in Tree Peony（Paeonia section Moutan）

ZHONG Yuan, DU Mingjie, CHENG Fangyun^*(), LI Kunyan, JI Runze, CUI Jun

Beijing Laboratory of Urban and Rural Ecological Environment，National Engineering Research Center for Floriculture，Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding，Peony International Institute，School of Landscape Architecture，Beijing Forestry University，Beijing 100083，China

Received:2023-02-24 Revised:2023-05-23 Online:2023-07-25 Published:2023-07-26
Contact: CHENG Fangyun

Abstract

Abstract:

Three allotetraploid tree peony materials were first found by karyotype analysis，and their fertility characteristics and breeding potentiality were analyzed based on hybridization test. The results showed that each of the three materials had 20 chromosomes，and the karyotype formulas were as follows：07-LB-30：2n = 4x = 20 = 16m（1SAT）+ 4st（1SAT），07-LB-22：2n = 4x = 20 = 15m（1SAT）+ 1sm （1SAT）+ 4st（3SAT），07-LB-56：2n = 4x = 20 = 16m（3SAT）+ 4st. The hybridization test showed that 07-LB-30 was a superior parent material with good fertility either as male or female parent. 07-LB-22 was an excellent male sterile female parent and of great value in breeding. 07-LB-56 was sterile as the female parent，but showed some fertility as male parent in hybridization，which leads it to an important material for breeding polyploid tree peony with double flower type.

Key words: Paeonia, section Moutan, P. × lemoinei, tetraploid, fertility

ZHONG Yuan, DU Mingjie, CHENG Fangyun, LI Kunyan, JI Runze, CUI Jun. Karyotype and Fertility Analysis of Allotetraploids in Tree Peony（Paeonia section Moutan）[J]. Acta Horticulturae Sinica, 2023, 50(7): 1444-1454.

Figures/Tables 8

References 43

[1]	Abbott R, Albach D, Ansell S, Arntzen J W, Baird S J E, Bierne N, Boughman J, Brelsford A, Buerkle C A, Buggs R, Butlin R K, Dieckmann U, Eroukhmanoff F, Grill A, Cahan S H, Hermansen J S, Hewitt G, Hudson A G, Jiggins C, Jones J, Keller B, Marczewski T, Mallet J, Martinez-Rodriguez P, Möst M, Mullen S, Nichols R, Nolte A W, Parisod C, Pfennig K, Rice A M, Ritchie M G, Seifert B, Smadja C M, Stelkens R, Szymura J M, Väinölä R, Wolf J B W, Zinner D. 2013. Hybridization and speciation. Journal of Evolutionary Biology, 26 (2):229-246. doi: 10.1111/j.1420-9101.2012.02599.x pmid: 23323997
[2]	Alix K, Gerard P R, Schwarzacher T, Heslop-Harrison J S. 2017. Polyploidy and interspecific hybridization:partners for adaptation,speciation and evolution in plants. Annals of Botany, 120 (2):183-194. doi: 10.1093/aob/mcx079 URL
[3]	Cao Xi-jun, Cheng Fang-yun. 2009. Primary introducing report on advanced generation hybrids of tree peonies//Advances in Ornamental Horticulture of China,2009. Hangzhou: Ornamental Horticulture Professional Committee of Chinese Society for Horticulture Science. (in Chinese)
	曹羲君, 成仿云. 2009. 牡丹高代杂种引种初报//中国观赏园艺研究进展 2009. 杭州:中国园艺学会观赏园艺专业委员会.
[4]	Chen L T, Liu Y G. 2014. Male sterility and fertility restoration in crops. Annual Review of Plant Biology, 65:579-606. doi: 10.1146/annurev-arplant-050213-040119 pmid: 24313845
[5]	Chen Z J. 2010. Molecular mechanisms of polyploidy and hybrid vigor. Trends in Plant Science, 15 (2):57-71. doi: 10.1016/j.tplants.2009.12.003 pmid: 20080432
[6]	Cheng F Y. 2007. Advances in the breeding of tree peonies and a cultivar system for the cultivar group. International Journal of Plant Breeding, 1 (2):89-104.
[7]	Cheng Fang-yun, Li Jia-jue. 1998. Exportation of Chinese tree peonies(Mudan)and their development in other countriesⅠ:cultivated. Journal of Northwest Normal University (Natural Science), 34 (1):112-119. (in Chinese)
	成仿云, 李嘉珏. 1998. 中国牡丹的输出及其在国外的发展Ⅰ:栽培牡丹. 西北师范大学学报(自然科学版), 34 (1):112-119.
[8]	Cheng Fang-yun, Li Jia-jue, Yu Ling. 1998. Exportation of Chinese tree peonies (Mudan) and their developments in other countries Ⅱ :wild species. Journal of Northwest Normal University (Natural Science), 34 (3):106-111. (in Chinese)
	成仿云, 李嘉珏, 于玲. 1998. 中国牡丹的输出及其在国外的发展Ⅱ:野生牡丹. 西北师范大学学报(自然科学版), 34 (3):106-111.
[9]	Gong Xun, Gu Zhi-jian, Wu Quan-an. 1991. A cytological study of seven populations in Paeonia delavayi var. lutea. Acta Botanica Yunnanica, 13 (4):402-410. (in Chinese)
	龚洵, 顾志建, 武全安. 1991. 黄牡丹七个居群的细胞学研究. 云南植物研究, 13 (4):402-410.
[10]	Han Xin. 2014. Studies on parental selection of tree peonies and genetic performance of several traits in F₁ hybrids[M. D. dissertation]. Beijing:Beijing Forestry University. (in Chinese)
	韩欣. 2014. 牡丹杂交亲本选择及F₁代遗传表现[硕士论文]. 北京: 北京林业大学.
[11]	Hao Q, Aoki N, Katayama J, Kako T, Cheon K S, Akazawa Y, Kobayashi N. 2013. Crossability of American tree peony‘High Noon’as seed parent with Japanese cultivars to breed superior cultivars. Euphytica, 191:35-44. doi: 10.1007/s10681-012-0853-3 URL
[12]	He Gui-mei, Cheng Fang-yun. 2006. Morphological observation of sexual reproduction abortion in‘High Noon’tree peony. Acta Horticulturae Sinica, 33 (3):660-663. (in Chinese)
	何桂梅, 成仿云. 2006. ‘正午’牡丹有性生殖败育的形态学观察. 园艺学报, 33 (3):660-663.
[13]	Hong D Y. 2010. Peonies of the World:Taxonomy and Phytogeography. UK: Kew Publishing.
[14]	Hong D Y. 2021. Peonies of the World:Phylogeny and Evolution. UK: Kew Publishing.
[15]	Hong De-yuan, Zhang Zhi-xian, Zhu Xiang-yun. 1988. Studies on the genus Paeonia (1) - report of karyotypes of some wild species in China. Acta Phytotaxonomica Sinica, 7 (4):33-43. (in Chinese)
	洪德元, 张志宪, 朱相云. 1988. 芍药属的研究 (1)——国产几个野生种核型的报道. 植物分类学报, 7 (4):33-43.
[16]	Hong De-yuan, Zhou Shi-liang, He Xing-jin, Yuan Jun-hui, Zhang Yan-long, Cheng Fang-yun, Zeng Xiu-li, Wang Yan, Zhang Xiu-xin. 2017. Current status of wild tree peony species with special reference to conservation. Biodiversity Science, 25 (7):781-793. (in Chinese) doi: 10.17520/biods.2017129
	洪德元, 周世良, 何兴金, 袁军辉, 张延龙, 成仿云, 曾秀丽, 王雁, 张秀新. 2017. 野生牡丹的生存状况和保护. 生物多样性, 25 (7):781-793. doi: 10.17520/biods.2017129
[17]	Ji J L, Huang J X, Yang L M, Fang Z Y, Zhang Y Y, Zhuang M, Lv H H, Wang Y, Liu Y M, Li Z S, Han F Q. 2020. Advances in research and application of male sterility in Brassica oleracea. Horticulturae, 6:101. doi: 10.3390/horticulturae6040101 URL
[18]	Li Mao-xue, Chen Rui-yang. 1985. A suggestion on the standardizaton of karyotype analysis in plants. Journal of Wuhan Botanical Research, 3 (4):297-302. (in Chinese)
	李懋学, 陈瑞阳. 1985. 关于植物核型分析的标准化问题. 武汉植物学研究, 3 (4):297-302.
[19]	Li Mao-xue, Zhang Xiao-fang. 1982. Cytogenetic observation of triploid tree peony. Hereditas, 4 (5):19-21. (in Chinese)
	李懋学, 张斅方. 1982. 三倍体牡丹的细胞遗传学观察. 遗传, 4 (5):19-21.
[20]	Liu Shu-min, Wang Lian-ying, Qin Kui-jie, Wu Di-xin. 1983. ‘Shou An Hong’,a triploid cultivar of tree peony (Paeonia suffruticosa). Journal of Beijing Forestry College,(4):62-67. (in Chinese)
	刘淑敏, 王莲英, 秦魁杰, 吴涤新. 1983. 牡丹三倍体品种——首案红. 北京林学院学报,(4):62-67.
[21]	Liu Xin. 2016. Studies on parental selection of tree peonies and early indentification of several hybrids[M. D. dissertation]. Beijing:Beijing Forestry University. (in Chinese)
	刘欣. 2016. 牡丹杂交亲本筛选及部分杂种苗SSR鉴定[硕士论文]. 北京: 北京林业大学.
[22]	Page M. 2005. The gardener’s peony:herbaceous and tree peonies. Portland · Cambridge: Timber Press.
[23]	Ramsey J, Schemske D W. 1998. Pathways,mechanisms,and rates of polyploid formation in flowering plants. Annual Review of Ecology & Systematics, 29 (1):467-501.
[24]	Salman-Minkov A, Sabath N, Mayrose I. 2016. Whole-genome duplication as a key factor in crop domestication. Nature Plants, 2:16115. doi: 10.1038/nplants.2016.115 pmid: 27479829
[25]	Sang T, Pan J, Zhang D M, Ferguson D, Wang C, Pan K Y, Hong D Y. 2004. Origins of polyploids:an example from peonies (Paeonia) and a model for angiosperms. Biological Journal of the Linnean Society, 82:561-571. doi: 10.1111/j.1095-8312.2004.00341.x URL
[26]	Saunders A P, Stebbins G L. 1938. Cytogenetic studies in Paeonia I. the compatibility of the species and the appearance of the hybrids. Genetics, 23 (1):65-82. doi: 10.1093/genetics/23.1.65 pmid: 17246877
[27]	Sodmergen. 2010. Sterility and fertility:keys for crop production in China. Science China-Life Sciences, 53 (12):1488-1489. doi: 10.1007/s11427-010-4094-1 URL
[28]	Stebbins G L. 1938. Cytogenetic studies in Paeonia II. the cytology of the diploid species and hybrids. Genetics, 23 (1):83-110. doi: 10.1093/genetics/23.1.83 pmid: 17246878
[29]	Storme N D, Mason A. 2014. Plant speciation through chromosome instability and ploidy change:cellular mechanisms,molecular factors and evolutionary relevance. Current Plant Biology, 1 (1):10-33. doi: 10.1016/j.cpb.2014.09.002 URL
[30]	Wang Lian-ying. 1997. Chinese tree peony. Beijing: China Forestry Publishing House. (in Chinese)
	王莲英. 1997. 中国牡丹品种图志. 北京: 中国林业出版社.
[31]	Wang Lian-ying, Yuan Tao. 2015. Sequel of Chinese tree peony. Beijing: China Forestry Publishing House. (in Chinese)
	王莲英, 袁涛. 2015. 中国牡丹品种图志(续志). 北京: 中国林业出版社.
[32]	Wang Lian-ying, Yuan Tao, Wang Fu, Li Qing-dao. 2013. The ex-situ conservation centre of Chinese Paeoniceae wild species and cultivation of new cultivars. Beijing: China Forestry Publishing House. (in Chinese)
	王莲英, 袁涛, 王福, 李清道. 2013. 中国芍药科野生种迁地保护与新品种培育. 北京: 中国林业出版社.
[33]	Wister J C. 1995. The peonies. Hopkins: The American Peony Society.
[34]	Wu Ting, Jia Ruidong, Yang Shuhua, Zhao Xin, Yu Xiaonan, Guo Yuan, Ge Hong. 2022. Research advances and prospects on Phalaenopsis polyploid breeding. Acta Horticulturae Sinica, 49 (2):448-462. (in Chinse) doi: 10.16420/j.issn.0513-353x.2020-0916
	吴婷, 贾瑞冬, 杨树华, 赵鑫, 于晓南, 国圆, 葛红. 2022. 蝴蝶兰多倍体育种研究进展与展望. 园艺学报, 49 (2):448-462. doi: 10.16420/j.issn.0513-353x.2020-0916
[35]	Xiao Jia-jia. 2010. A study on the crossing compatibility and hybrid abortion of Paeonia[M. D. dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	肖佳佳. 2010. 芍药属杂交亲和性及杂种败育研究[硕士论文]. 北京: 北京林业大学.
[36]	Xie L H, Niu L X, Zhang Y L, Jin M, Ji D, Zhang X X. 2017. Pollen sources influence the traits of seed and seed oil in Paeonia ostii‘Feng Dan’. Hortscience, 52 (5):700-705. doi: 10.21273/HORTSCI11803-17 URL
[37]	Yang Qiong-liang, Ou-yang Ting, Yan Hong, Yang Jing, Li Ling, Xia Xin-hua. 2015. Research progress of pollen allergy. Chinese Agricultural Science Bulletin, 31 (24):163-167. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb15040195
	杨琼梁, 欧阳婷, 颜红, 杨晶, 李玲, 夏新华. 2015. 花粉过敏的研究进展. 中国农学通报, 31 (24):163-167. doi: 10.11924/j.issn.1000-6850.casb15040195
[38]	Younis A, Hwang Y, Lim K. 2014. Exploitation of induced 2n-gametes for plant breeding. Plant Cell Reports, 33 (2):215-223. doi: 10.1007/s00299-013-1534-y pmid: 24311154
[39]	Zhang K, Wang X, Cheng F. 2019. Plant polyploidy:origin,evolution,and its influence on crop domestication. Horticultural Plant Journal, 5 (6):231-239. doi: 10.1016/j.hpj.2019.11.003
[40]	Zhao Na. 2014. Study on morphology and chromosome karyotype of distant hybridization progenies and their parents of tree peony [M. D. dissertation]. Beijing:Beijing Forestry University. (in Chinese)
	赵娜. 2014. 牡丹远缘杂交后代及亲本形态与核型研究[硕士论文]. 北京: 北京林业大学.
[41]	Zhong Yuan, Du Ming-jie, Liu Yu-xin, Cheng Fang-yun. 2019. Karyotype analysis and meiotic chromosome behavior observation on Paeonia × lemoinei‘High Noon’. Journal of Beijing Forestry University, 41 (10):68-73. (in Chinese)
	钟原, 杜明杰, 刘羽心, 成仿云. 2019. ‘正午’牡丹核型分析及减数分裂的染色体行为观察. 北京林业大学学报, 41 (10):68-73.
[42]	Zhou S L, Xu C, Liu J, Yu Y, Wu P, Cheng T, Hong D Y. 2021. Out of the Pan-Himalaya:Evolutionary history of the Paeoniaceae revealed by phylogenomics. Journal of Systematics and Evolution, 59 (6):1170-1182. doi: 10.1111/jse.v59.6 URL
[43]	Zhou S L, Zou X H, Zhou Z Q, Liu J, Xu C, Yu J, Wang Q, Zhang D M, Wang X Q, Ge S, Sang T, Pan K Y, Hong D Y. 2014. Multiple species of wild tree peonies gave rise to the‘King of flowers’,Paeonia suffruticosa Andrews. Proceedings of the Royal Society B: Biological Sciences, 281:20141687.

材料Material	雌蕊形态 Pistil morphology	心皮数Carpel number	花药形态Anther form	花粉量Pollen quantity
07-LB-30	正常Normal	3 ~ 5	大而饱满Big and full	多Abundant
07-LB-22	正常Normal	3 ~ 5	干瘪Wizened	无None
07-LB-56	畸形Abnormal	> 5	瘦小Thin and small	少A little

材料Material	雌蕊形态 Pistil morphology	心皮数Carpel number	花药形态Anther form	花粉量Pollen quantity
07-LB-30	正常Normal	3 ~ 5	大而饱满Big and full	多Abundant
07-LB-22	正常Normal	3 ~ 5	干瘪Wizened	无None
07-LB-56	畸形Abnormal	> 5	瘦小Thin and small	少A little

染色体序号 Chromosome No.	07-LB-30			07-LB-22			07-LB-56
染色体序号 Chromosome No.	相对长度/% Relative length	臂比 Arm ratio	类型 Type	相对长度/% Relative length	臂比 Arm ratio	类型 Type	相对长度/% Relative length	臂比 Arm ratio	类型 Type
1	6.27 ± 0.34	1.39 ± 0.14	m	6.19 ± 0.28	1.44 ± 0.30	m	5.88 ± 0.19	1.47 ± 0.10	m
2	6.02 ± 0.28	1.13 ± 0.15	m	5.93 ± 0.26	1.36 ± 0.15	m	5.67 ± 0.10	1.20 ± 0.13	m
3	5.82 ± 0.14	1.23 ± 0.19	m	5.80 ± 0.21	1.15 ± 0.11	m	5.63 ± 0.11	1.53 ± 0.17	m
4	5.66 ± 0.18	1.21 ± 0.15	m	5.70 ± 0.16	1.51 ± 0.29	m	5.54 ± 0.06	1.22 ± 0.17	m
5	5.53 ± 0.11	1.61 ± 0.23	m	5.56 ± 0.13	1.22 ± 0.15	m	5.47 ± 0.05	1.19 ± 0.09	m
6	5.43 ± 0.13	1.24 ± 0.18	m	5.49 ± 0.14	1.32 ± 0.26	m	5.41 ± 0.05	1.13 ± 0.09	m
7	5.24 ± 0.10	1.60 ± 0.23	m	5.37 ± 0.09	1.22 ± 0.16	m	5.34 ± 0.11	1.36 ± 0.18	m
8	5.19 ± 0.11	1.21 ± 0.20	m	5.25 ± 0.07	1.44 ± 0.27	m	5.32 ± 0.11	1.12 ± 0.06	m
9	5.06 ± 0.12	1.60 ± 0.18	m	5.15 ± 0.08	1.28 ± 0.20	m	5.20 ± 0.08	1.27 ± 0.07	m
10	5.05 ± 0.11	1.27 ± 0.23	m	5.06 ± 0.08	1.24 ± 0.14	m	5.16 ± 0.10	1.11 ± 0.07	m
11	4.92 ± 0.11	1.32 ± 0.25	m	4.99 ± 0.05	1.35 ± 0.24	m	5.08 ± 0.09	1.37 ± 0.22	m
12	4.86 ± 0.12	1.30 ± 0.22	m	4.93 ± 0.06	1.25 ± 0.19	m	4.98 ± 0.07	1.48 ± 0.16	m
13	4.78 ± 0.12	1.48 ± 0.27	m	4.87 ± 0.09	1.50 ± 0.19	m	4.90 ± 0.06	1.55 ± 0.11	m^*
14	4.68 ± 0.10	1.25 ± 0.26	m	4.66 ± 0.13	1.50 ± 0.18	m	4.84 ± 0.14	1.14 ± 0.11	m^*
15	4.63 ± 0.17	1.58 ± 0.23	m	4.50 ± 0.13	1.74 ± 0.13	sm^*	4.75 ± 0.13	1.50 ± 0.16	m
16	4.47 ± 0.16	1.57 ± 0.15	m^*	4.41 ± 0.16	1.36 ± 0.18	m^*	4.50 ± 0.22	1.56 ± 0.13	m^*
17	4.30 ± 0.21	4.11 ± 0.50	st^*	4.25 ± 0.21	3.78 ± 0.40	st^*	4.23 ± 0.12	3.71 ± 0.18	st
18	4.17 ± 0.15	3.97 ± 0.52	st	4.11 ± 0.16	4.04 ± 0.52	st	4.19 ± 0.17	4.11 ± 0.30	st
19	4.03 ± 0.15	3.49 ± 0.36	st	3.96 ± 0.11	3.81 ± 0.45	st^*	3.94 ± 0.21	3.90 ± 0.40	st
20	3.88 ± 0.17	3.63 ± 0.19	st	3.83 ± 0.27	3.58 ± 0.48	st^*	3.94 ± 0.14	3.47 ± 0.36	st

染色体序号 Chromosome No.	07-LB-30			07-LB-22			07-LB-56
染色体序号 Chromosome No.	相对长度/% Relative length	臂比 Arm ratio	类型 Type	相对长度/% Relative length	臂比 Arm ratio	类型 Type	相对长度/% Relative length	臂比 Arm ratio	类型 Type
1	6.27 ± 0.34	1.39 ± 0.14	m	6.19 ± 0.28	1.44 ± 0.30	m	5.88 ± 0.19	1.47 ± 0.10	m
2	6.02 ± 0.28	1.13 ± 0.15	m	5.93 ± 0.26	1.36 ± 0.15	m	5.67 ± 0.10	1.20 ± 0.13	m
3	5.82 ± 0.14	1.23 ± 0.19	m	5.80 ± 0.21	1.15 ± 0.11	m	5.63 ± 0.11	1.53 ± 0.17	m
4	5.66 ± 0.18	1.21 ± 0.15	m	5.70 ± 0.16	1.51 ± 0.29	m	5.54 ± 0.06	1.22 ± 0.17	m
5	5.53 ± 0.11	1.61 ± 0.23	m	5.56 ± 0.13	1.22 ± 0.15	m	5.47 ± 0.05	1.19 ± 0.09	m
6	5.43 ± 0.13	1.24 ± 0.18	m	5.49 ± 0.14	1.32 ± 0.26	m	5.41 ± 0.05	1.13 ± 0.09	m
7	5.24 ± 0.10	1.60 ± 0.23	m	5.37 ± 0.09	1.22 ± 0.16	m	5.34 ± 0.11	1.36 ± 0.18	m
8	5.19 ± 0.11	1.21 ± 0.20	m	5.25 ± 0.07	1.44 ± 0.27	m	5.32 ± 0.11	1.12 ± 0.06	m
9	5.06 ± 0.12	1.60 ± 0.18	m	5.15 ± 0.08	1.28 ± 0.20	m	5.20 ± 0.08	1.27 ± 0.07	m
10	5.05 ± 0.11	1.27 ± 0.23	m	5.06 ± 0.08	1.24 ± 0.14	m	5.16 ± 0.10	1.11 ± 0.07	m
11	4.92 ± 0.11	1.32 ± 0.25	m	4.99 ± 0.05	1.35 ± 0.24	m	5.08 ± 0.09	1.37 ± 0.22	m
12	4.86 ± 0.12	1.30 ± 0.22	m	4.93 ± 0.06	1.25 ± 0.19	m	4.98 ± 0.07	1.48 ± 0.16	m
13	4.78 ± 0.12	1.48 ± 0.27	m	4.87 ± 0.09	1.50 ± 0.19	m	4.90 ± 0.06	1.55 ± 0.11	m^*
14	4.68 ± 0.10	1.25 ± 0.26	m	4.66 ± 0.13	1.50 ± 0.18	m	4.84 ± 0.14	1.14 ± 0.11	m^*
15	4.63 ± 0.17	1.58 ± 0.23	m	4.50 ± 0.13	1.74 ± 0.13	sm^*	4.75 ± 0.13	1.50 ± 0.16	m
16	4.47 ± 0.16	1.57 ± 0.15	m^*	4.41 ± 0.16	1.36 ± 0.18	m^*	4.50 ± 0.22	1.56 ± 0.13	m^*
17	4.30 ± 0.21	4.11 ± 0.50	st^*	4.25 ± 0.21	3.78 ± 0.40	st^*	4.23 ± 0.12	3.71 ± 0.18	st
18	4.17 ± 0.15	3.97 ± 0.52	st	4.11 ± 0.16	4.04 ± 0.52	st	4.19 ± 0.17	4.11 ± 0.30	st
19	4.03 ± 0.15	3.49 ± 0.36	st	3.96 ± 0.11	3.81 ± 0.45	st^*	3.94 ± 0.21	3.90 ± 0.40	st
20	3.88 ± 0.17	3.63 ± 0.19	st	3.83 ± 0.27	3.58 ± 0.48	st^*	3.94 ± 0.14	3.47 ± 0.36	st

母本 Female parent	父本 Male parent	授粉花朵数 Number of pollinated flowers	结实粒数 Number of plump seeds	每朵结实粒数 Number of plump seeds per pollinated flower	杂交年份 Hybrid year
07-LB-30	07-LB-X6 + 07-LB-33	47	274	5.83	2020
07-LB-22	07-LB-30	7	30	4.29	2019
	07-LB-X6 + 07-LB-30	42	253	6.02	2020
	小计Subtotal	49	283	5.78
07-LB-56	07-LB-X6 + 07-LB-30	30	9	0.30	2020

Karyotype and Fertility Analysis of Allotetraploids in Tree Peony（Paeonia section Moutan）

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父本 Male parent	母本（芍药） Female parent（Paeonia lactiflora）	授粉花朵数 Number of pollinated flowers	结实粒数 Number of plump seeds	每朵结实粒数 Number of plump seeds per pollinated flower	杂交年份 Hybrid year
07-LB-30	0-2	8	25	3.13	2018
	0-3	8	37	4.63	2019
	2-17	15	29	1.93	2019
	2-17	41	163	3.98	2020
	Martha W.	23	199	8.65	2018
	Martha W.	10	113	11.30	2019
	Martha W.	13	108	8.31	2020
	白色重瓣芍药White double strain	18	116	6.44	2018
	白色重瓣芍药White double strain	31	55	1.77	2020
	粉色半重瓣芍药Pink semi-double strain	17	71	4.18	2019
	紫色单瓣芍药 Purple single strain	18	57	3.17	2020
	小计Subtotal	202	973	4.82
07-LB-56	10-1-4	4	4	1.00	2018
	Martha W.	12	66	5.50	2019
	Martha W.	18	50	2.78	2020
	小计Subtotal	34	120	3.53

母本 Female parent	授粉花朵数 Number of pollinated flowers	结实粒数 Number of plump seeds	每朵结实粒数 Number of plump seeds per pollinated flower	杂交年份 Hybrid year
07-LB-X6	15	60	4.00	2018
07-LB-X6	8	10	1.25	2019
07-LB-X9	9	92	10.22	2018
07-LB-6	12	58	4.83	2018
07-LB-6	6	11	1.83	2019
07-LB-8	3	2	0.67	2019
07-LB-12	12	58	4.83	2018
07-LB-12	6	1	0.17	2019
07-LB-24	9	2	0.22	2019
07-LB-33	11	9	0.82	2019
07-LB-43	14	37	2.64	2018
07-LB-43	11	37	3.36	2019
07-LB-62	14	95	6.79	2018
07-LB-62	6	11	1.83	2019
合计Total	136	483	3.55

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