Abstract: PhTs2，a gene related to flower development，was cloned from Petunia hybrida‘Fantasy Red’. The CDS of this gene is 855 bp long and encodes 284 amino acids with a conserved adh-short domain. The phylogenetic analysis showed that the PhTs2 protein was closely related to the homologous proteins in tobacco and tomato. Quantitative real-time PCR analysis indicated that the gene was specifically expressed in the early developmental stage of anther in petunia. To analyze its function，an overexpression vector of PhTs2 was constructed and transformed into tobacco，then the transgenic tobaccos were identified：Phenotypic observation of transgenic tobaccos showed abnormal anther morphology and reduced pollen count；pollen vigor and germination rate were extremely reduced；the malformed pollen grains with irregular outer wall texture was observed by scanning electron microscopy；the semi-thin section showed the disorder of the tapetum development and the abnormal morphology of pollen grains. This study provides potential genetic resources for the cultivation of male sterile lines.

Key words: Petunia hybrida, fertility；anther, tapetum, male sterility, pollen