Identification of Anthocyanin Compositions and Expression Analysis of Key Related Genes in Fragaria × ananassa

doi:10.16420/j.issn.0513-353x.2022-0066

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (4): 791-801.doi: 10.16420/j.issn.0513-353x.2022-0066

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Identification of Anthocyanin Compositions and Expression Analysis of Key Related Genes in Fragaria × ananassa

YUAN Huazhao, PANG Fuhua, WANG Jing, CAI Weijian, XIA Jin, ZHAO Mizhen()

Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement，Institute of Pomology，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China

Received:2022-11-28 Revised:2023-03-07 Online:2023-04-25 Published:2023-04-27
Contact: *（E-mail：njzhao@163.com）

Abstract

Abstract:

HPLC-MS/MS was applied to establish a method to isolate and identify anthocyanins in Fragaria × ananassa fruit，and anthocyanins from 12 strawberry cultivars with different fruit colors were analyzed qualitatively and quantitatively. Meanwhile，a comparative analysis was made on the number and chromosome location of genes related to anthocyanin synthesis between diploid(F. vesca)and octoploid(F. × ananassa)strawberry genome，and the transcription levels of which during fruit development were analyzed by RNA-Seq and qRT-PCR. In this study，totally eight anthocyanin components were detected，and peonidin-3-O-glucoside，peonidin-malonylglucoside and peonidin-3-methylmalonylglucoside were first detected in F. × ananassa. Pelargonidin-3-glucoside was the main anthocyanin in all 12 strawberry cultivars，while the total contents were different. Seventy-three genes related to anthocyanin synthesis were identified in the F. × ananassa genome distributed uniformly in four subgenomes，which were almost three-or four-fold than in the F. vesca genome. The results of RNA-Seq showed that on the whole there was no significant difference and biased expression between the expression levels of multi-copy genes in F.×ananassa fruit. The expression levels of key structural genes for anthocyanin synthesis(PAL1,CHS,CHI,F3H,DFR1,ANS,UFGT),transportation(GST)and transcription regulatory factor MYB10 were significantly increased during fruit development，especially during anthocyanin accumulation stages，suggesting that these genes play key roles in anthocyanin accumulation in strawberry fruits.

Key words: Fragaria × ananassa, fruit, color, anthocyanin, polyploid

CLC Number:

S668.1

YUAN Huazhao, PANG Fuhua, WANG Jing, CAI Weijian, XIA Jin, ZHAO Mizhen. Identification of Anthocyanin Compositions and Expression Analysis of Key Related Genes in Fragaria × ananassa[J]. Acta Horticulturae Sinica, 2023, 50(4): 791-801.

Figures/Tables 8

References 25

[1]	Aharoni A, de Vos C H, Wein M, Sun Z, Greco R, Kroon A, Mol J N, O'Connell A P. 2001. The strawberry FaMYB 1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant Journal, 28 (3):319-332. doi: 10.1046/j.1365-313x.2001.01154.x pmid: 11722774
[2]	Edger P P, Poorten T J, van Buren R, Hardigan M A, Colle M, McKain M R, Smith R D, Teresi S J, Nelson A D L, Wai C M, Alger E I, Bird K A, Yocca A E, Pumplin N, Ou S, Ben-Zvi G, Brodt A, Baruch K, Swale T, Shiue L. 2019. Origin and evolution of the octoploid strawberry genome. Nature Genetics, 51:541-547. doi: 10.1038/s41588-019-0356-4 pmid: 30804557
[3]	Espley R V, Hellens R P, Putterill J, Stevenson D E, Kutty-Amma S, Allan A C. 2007. Red colouration in apple fruit is due to the activity of the MYB transcription factor,MdMYB10. The Plant Journal, 49:414-427. doi: 10.1111/tpj.2007.49.issue-3 URL
[4]	Gao Q, Luo H F, Li Y P, Liu Z C, Kang C Y. 2020. Genetic modulation of RAP alters fruit coloration in both wild and cultivated strawberry. Plant Biotechnol J, 18 (7):1550-1561. doi: 10.1111/pbi.13317 pmid: 31845477
[5]	Griesser M, Hoffmann T, Bellido M L, Rosati C, Fink B, Kurtzer R, Aharoni A, Muñoz-Blanco J, Schwab W. 2008. Redirection of flavonoid biosynthesis through the down-regulation of an anthocyanidin glucosyltransferase in ripening strawberry fruit. Plant Physiology, 146 (4):1528-1539. doi: 10.1104/pp.107.114280 pmid: 18258692
[6]	Han Hai-hua, Liang Ming-zhi, Wang Li, Luo Qiong-xian, Zhao Tian-tian. 2011. Research progress of anthocyanin. Journal of Tea, 37 (4):217-220. (in Chinese)
	韩海华, 梁名志, 王丽, 罗琼仙, 赵甜甜. 2011. 花青素的研究进展及其应用. 茶叶, 37 (4):217-220.
[7]	Hegarty M J, Barker G L, Wilson I D, Abbott R J, Edwards K J, Hiscock S J. 2006. Transcriptome shock after interspecific hybridization in senecio is ameliorated by genome duplication. Current Biology, 16:1652-1659. doi: 10.1016/j.cub.2006.06.071 pmid: 16920628
[8]	Hong Yanhong, Ye Qinghua, Li Zekun, Wang Wei, Xie Qian, Chen Qingxi, Chen Jianqing. 2021. Accumulation of anthocyanins in red-flowered strawberry‘Meihong’petals and expression analysis of MYB gene. Acta Horticulturae Sinica, 48 (8):1470-1484. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0477
	洪燕红, 叶清华, 李泽坤, 王威, 谢倩, 陈清西, 陈建清. 2021. 红花草莓‘莓红’花瓣花色苷积累及其MYB基因的表达分析. 园艺学报, 48 (8):1470-1484. doi: 10.16420/j.issn.0513-353x.2020-0477
[9]	Jiang Wei-wei, Ren Guo-feng. 2009. Review on anti-tumor effect of anthocyanin. Food Science, 30 (9):281-284. (in Chinese) doi: 10.7506/spkx1002-6630-200909066
	姜伟伟, 任国峰. 2009. 花色苷的抗肿瘤效应研究进展. 食品科学, 30 (9):281-284.
[10]	Li Lixian, Wang Shuo, Chen Ying, Wu Yingtao, Wang Yaqian, Fang Yue, Chen Xuesen, Tian Changping, Feng Shouqian. 2022. PavMYB10.1 promotes anthocyanin accumulation by positively regulating pavriant in sweet cherry. Acta Horticulturae Sinica, 49 (5):1023-1030. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0732
	李丽仙, 王烁, 陈莹, 邬滢涛, 王雅倩, 房月, 陈学森, 田长平, 冯守千. 2022. 甜樱桃PavMYB10.1促进PavRiant表达和花青苷积累. 园艺学报, 49 (5):1023-1030. doi: 10.16420/j.issn.0513-353x.2021-0732
[11]	Lin B W, Gong C C, Song H F, Cui Y Y. 2017. Effects of anthocyanins on the prevention and treatment of cancer. British Journal of Pharmacology, 174 (11):1126-1128.
[12]	Liu Jian-li, Liu Xiao, Cao Xiang-yu, Yu Hui, Yang Si-min, Sun Yu-hang. 2015. Purification and in vitro antioxidant activity of anthocyanins from Padus racemose. Food Science, 36 (15):5-10. (in Chinese) doi: 10.1111/jfds.1971.36.issue-1 URL
	刘剑利, 刘晓, 曹向宇, 于慧, 杨思敏, 孙宇航. 2015. 稠李花色苷的纯化及体外抗氧化活性. 食品科学, 36 (15):5-10. doi: 10.7506/spkx1002-6630-201515002
[13]	Lopes-da-Silva F, de Pascual-Teresa S, Rivas-Gonzalo J. 2002. Identification of anthocyanin pigments in strawberry(cv Camarosa)by LC using DAD and ESI-MS detection. European Food Research and Technology, 214:248-253. doi: 10.1007/s00217-001-0434-5 URL
[14]	Lopes-da-Silva F, Escribano-Bailón M T, Pérez-Alonso J J, Rivas-Gonzalo J, Santos-Buelga C. 2007. Anthocyanin pigments in strawberry. Food Science and Technology, 40 (2):374-382. doi: 10.1590/fst.17419 URL
[15]	Luo Yun, Chen Zong-ling, Song Wei-tang, Wang Hong-qing. 2014. Identification and analysis for anthocyanins compositions in strawberry. Journal of China Agricultural Aniversity, 19 (5):86-94. (in Chinese)
	罗赟, 陈宗玲, 宋卫堂, 王红清. 2014. 草莓果实花色苷成分组成鉴定及分析. 中国农业大学学报, 19 (5):86-94.
[16]	Njuguna W, Liston A, Cronn R, Ashman T L, Bassil N. 2013. Insights into phylogeny,sex function and age of Fragaria based on whole chloroplast genome sequencing. Mol Phylogenet Evol, 66:17-29. doi: 10.1016/j.ympev.2012.08.026 pmid: 22982444
[17]	Opes-da-Silva F, de Pascual-Teresa S, Rivas-Gonzalo J, Santos-Buelga C. 2002. Identification of anthocyanin pigments in strawberry(cv Camarosa) by LC using DAD and ESI-MS detection. European Food Research and Technology, 214:248-253. doi: 10.1007/s00217-001-0434-5 URL
[18]	Paolocci F, Robbins M P, Passeri V, Hauck B, Morris P, Rubini A, Arcioni S, Damiani F. 2010. The strawberry transcription factor FaMYB1inhibits the biosynthesis of proanthocyanidins in Lotus corniculatus leaves. J Exp Bot, 62 (3):1189-1200. doi: 10.1093/jxb/erq344 URL
[19]	Sun J H, Liu X J, Yang T B, Yang T B, Slovin J, Chen P. 2014. Profiling polyphenols of two diploid strawberry(Fragaria vesca)inbred lines using UHPLC-HRMS. Food Chemistry, 146:289-298. doi: 10.1016/j.foodchem.2013.08.089 URL
[20]	Wang H, Zhang H, Yang Y, Li M, Zhang Y, Liu J, Dong J, Li J, Butelli E, Xue Z, Wang A, Wang G, Martin C, Jin W. 2020. The control of red colour by a family of MYB transcription factors in octoploid strawberry(Fragaria × ananassa)fruits. Plant Biotechnol Journal, 18:1169-1184. doi: 10.1111/pbi.v18.5 URL
[21]	Wang Z G, Meng D, Wang A D, Li T L, Jiang S L, Cong P H, Li T Z. 2013. The methylation of the PcMYB 10 promoter is associated with green-skinned sport in max red bartlett pear. Plant Physiology, 162 (2):885-896. doi: 10.1104/pp.113.214700 URL
[22]	Yang Xiaofang, Zhang Zuying, Miao Lixiang, Zhang Yuchao, Shen Lan, Qin Qiaoping, Jiang Guihua. 2020. Preliminary analysis on the molecular differences of fruit colouring between yuexin strawberry and its somaclonal mutation. Acta Horticulturae Sinica, 47 (10):1999-2008. (in Chinese) doi: 10.16420/j.issn.0513-353x.2019-0996 URL
	杨肖芳, 张祖瑛, 苗立祥, 张豫超, 沈岚, 秦巧平, 蒋桂华. 2020. ‘越心’草莓组培突变体着色差异的分子机理初探. 园艺学报, 47 (10):1999-2008.
[23]	Yuan H Z, Pang F H, Cai W J, Chen X D, Zhao M Z, Yu H M. 2021. Genome-wide analysis of the invertase genes in strawberry(Fragaria × ananassa). Journal of Integrative Agriculture, 20 (10):2652-2665. doi: 10.1016/S2095-3119(20)63381-0 URL
[24]	Zhang Qiong, Wang Hong-qing, Leng Ping, Jia Le-xin. 2008. Mechanism of anthocyanins and flavonols in fruit development of strawberries. Journal of China Agricultural Aniversity, 35 (12):1735-1741. (in Chinese)
	张琼, 王红清, 冷平, 贾乐新. 2008. 草莓果实发育过程中花色苷和黄酮醇类物质的形成机制. 园艺学报, 35 (12):1735-1741.
[25]	Zhao Mi-zhen, Wang Jin, Wang Zhuang-wei, Qian Ya-ming, Wu Wei-min. 2012. Development status of strawberry industry in the world and sustainable development countermeasures of Strawberry industry in Jiangsu,Zhejiang and Shanghai. Jiangsu Agricultural Sciences, 40 (2):1-3. (in Chinese)
	赵密珍, 王静, 王壮伟, 钱亚明, 吴伟民. 2012. 世界草莓产业发展现状及江浙沪草莓产业可持续发展对策. 江苏农业科学, 40 (2):1-3.

基因Gene	正向引物（5′-3′）Forword primer	反向引物（5′-3′）Reverse primer
FaPAL1	CAGTGACCAACCATGTCCAG	TCCTCCAAATGCCTCAAATC
FaCHS	GCCTGAGAAGTTAGAAGCCACG	CGAACCCAAACAGAACACCC
FaCHI	AGCATCACCCTCTACCCTCAT	ACCCACTGCACCCATAGCTG
FaF3H	TGTGGCGTTTGAGTCCGAGA	TGACGAGCTGATGGGGTTGG
FaDFR1	CAGGGTTTGAGTTCAAGTACAGC	TATCGCCATTCTCCTGCTTCT
FaANS	ATCTTCTCCTTGGGCGGCTC	AACATGGTTCCCGGTCTGCA
FaUFGT	CAAGCAATCCAACAGCTCAATC	GAAAACATACCCCTCCGGCAC
FaGST	CAAGTTCCAGCAATCGAAGA	TGGGAAGGATCACAAGTTGA
FaMYB10	GAGAAGGCAAATGGCATCAT	TTCATCCTCTGCAAACTCTCC

基因Gene	正向引物（5′-3′）Forword primer	反向引物（5′-3′）Reverse primer
FaPAL1	CAGTGACCAACCATGTCCAG	TCCTCCAAATGCCTCAAATC
FaCHS	GCCTGAGAAGTTAGAAGCCACG	CGAACCCAAACAGAACACCC
FaCHI	AGCATCACCCTCTACCCTCAT	ACCCACTGCACCCATAGCTG
FaF3H	TGTGGCGTTTGAGTCCGAGA	TGACGAGCTGATGGGGTTGG
FaDFR1	CAGGGTTTGAGTTCAAGTACAGC	TATCGCCATTCTCCTGCTTCT
FaANS	ATCTTCTCCTTGGGCGGCTC	AACATGGTTCCCGGTCTGCA
FaUFGT	CAAGCAATCCAACAGCTCAATC	GAAAACATACCCCTCCGGCAC
FaGST	CAAGTTCCAGCAATCGAAGA	TGGGAAGGATCACAAGTTGA
FaMYB10	GAGAAGGCAAATGGCATCAT	TTCATCCTCTGCAAACTCTCC

组分 Peak No.	保留时间/min Retention time	MS[M+H]/（m/z）	MS2/（m/z）	化合物 Compound
1	15.47	449	287	矢车菊素-3-葡糖苷^* Cyanidin-3-glucoside^*
2	16.86	433	271	天竺葵素-3-葡糖苷^* Pelargonidin-3-glucoside^*
3	17.73	463	301，286	芍药素-3-葡糖苷Peonidin-3-O-glucoside
4	19.12	535	287	矢车菊素-丙二酰葡糖苷Cyanidin- malonylglucoside
5	20.23	519	271	天竺葵素-丙二酰葡糖苷Pelargonidin-malonylglucoside
6	20.73	549	301，286	芍药素-丙二酰葡糖苷Peonidin-malonylglucoside
7	21.74	533	271	天竺葵素-3-甲基丙二酰基葡糖苷Pelargonidin-3-methylmalonylglucoside
8	22.05	563	301，286	芍药素-3-甲基丙二酰基葡糖苷Peonidin-3-methylmalonylglucoside

组分 Peak No.	保留时间/min Retention time	MS[M+H]/（m/z）	MS2/（m/z）	化合物 Compound
1	15.47	449	287	矢车菊素-3-葡糖苷^* Cyanidin-3-glucoside^*
2	16.86	433	271	天竺葵素-3-葡糖苷^* Pelargonidin-3-glucoside^*
3	17.73	463	301，286	芍药素-3-葡糖苷Peonidin-3-O-glucoside
4	19.12	535	287	矢车菊素-丙二酰葡糖苷Cyanidin- malonylglucoside
5	20.23	519	271	天竺葵素-丙二酰葡糖苷Pelargonidin-malonylglucoside
6	20.73	549	301，286	芍药素-丙二酰葡糖苷Peonidin-malonylglucoside
7	21.74	533	271	天竺葵素-3-甲基丙二酰基葡糖苷Pelargonidin-3-methylmalonylglucoside
8	22.05	563	301，286	芍药素-3-甲基丙二酰基葡糖苷Peonidin-3-methylmalonylglucoside

品种 Cultivar	各组分占比/% Percentage of each anthocyanin component
品种 Cultivar	1	2	3	4	5	6	7	8
四季香 Sijixiang	4.25 ± 0.18	81.11 ± 3.42	6.05 ± 0.26	ND	8.59 ± 0.36	ND	ND	ND
明旭Mingxu	3.32 ± 0.14	64.02 ± 2.71	6.15 ± 0.26	0.82 ± 0.03	22.93 ± 0.97	ND	2.76 ± 0.12	ND
97-17-1	5.47 ± 0.17	75.19 ± 2.28	7.18 ± 0.22	ND	12.16 ± 0.37	ND	ND	ND
97-4-17	5.11 ± 0.31	75.84 ± 4.54	8.52 ± 0.51	ND	10.53 ± 0.63	ND	ND	ND
甜查理 Sweet Charlie	3.24 ± 0.17	70.65 ± 3.66	10.07 ± 0.52	ND	12.35 ± 0.64	ND	3.69 ± 0.19	ND
幸香 Sachinoka	6.80 ± 0.49	79.23 ± 5.73	7.66 ± 0.55	ND	ND	0.87 ± 0.06	ND	5.44 ± 0.39
久59-1 Jiu 59-1	11.21 ± 0.69	78.46 ± 4.86	9.28 ± 0.57	ND	ND	1.05 ± 0.06	ND	ND
宁丰 Ningfeng	9.83 ± 0.43	75.53 ± 3.29	14.64 ± 0.64	ND	ND	ND	ND	ND
紫金久红 Zijin Jiuhong	9.97 ± 0.59	83.17 ± 4.96	ND	ND	ND	ND	ND	6.86 ± 0.41
香Xiang	10.54 ± 0.52	76.47 ± 3.75	12.99 ± 0.64	ND	ND	ND	ND	ND
枥木少女 Tochiotome	14.13 ± 0.92	72.38 ± 4.71	13.49 ± 0.88	1.23 ± 0.08	ND	ND	ND	ND
桃熏Tokun	14.00 ± 0.90	49.64 ± 3.20	15.27 ± 0.99	ND	21.09 ± 1.36	ND	ND	ND
平均值 Average	8.16 ± 0.46	73.47 ± 3.93	10.12 ± 0.50	1.03 ± 0.06	14.61 ± 0.72	0.96 ± 0.06	3.23 ± 0.15	6.15 ± 0.04

Identification of Anthocyanin Compositions and Expression Analysis of Key Related Genes in Fragaria × ananassa

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