https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 378-394.doi: 10.16420/j.issn.0513-353x.2020-0946

• Research Papers • Previous Articles     Next Articles

Effects of Exogenous Spermidine on Photosynthetic Characteristics and Gene Expression of Key Enzymes Under Salt Stress in Tea Plant

YANG Ni1, WAN Qiwen1, LI Yimin1, HAN Miaohua1, TENG Ruimin1, LIU Jiexia2, ZHUANG Jing1,*()   

  1. 1Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China,Tea Research Institute,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
    2State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-05-17 Revised:2021-10-23 Online:2022-02-25 Published:2022-02-28
  • Contact: ZHUANG Jing E-mail:zhuangjing@njau.edu.cn

Abstract:

In order to explore the regulatory effects of exogenous spermidine(Spd)on the photosynthetic characteristics under salt stress of tea plant,tea variety‘Zhongcha 108’was used as the experimental material in this study. Through different concentrations(0,0.5,1.0,1.5 mmol · L-1)Spd spray treatments,the effects of exogenous Spd on tea photosynthetic parameters,chlorophyll content,stomatal aperture and the expression levels of key enzyme genes under salt stress(200 mmol · L-1 NaCl)were detected and analyzed. The results showed that the net photosynthetic rate(Pn),transpiration rate(Tr),and chlorophyll content were significantly reduced,and the intercellular CO2 concentration(Ci)was increased under salt stress. Stomatal aperture was promoted the initial treatment(1,3 d),and inhibited gradually in the later period(5,9 d). After spraying exogenous Spd,Pn,Tr,stomatal conductance(Gs),chlorophyll a,chlorophyll b and Chlorophyll(a + b)contents were significantly increased under salt stress. Exogenous Spd can induce the up-regulation of CsRbcL,CsTK,CsFBPase,CsPRK and other photosynthetic carbon assimilation genes to varying degrees,and alleviate the influence of salt stress on the inhibition most chlorophyll synthesis genes. The study demonstrated that spraying exogenous Spd can effectively alleviate the degradation of chlorophyll,promote photosynthesis,and improve the salt tolerance of tea plant,of which 0.5 mmol · L-1 Spd was the most effective.

Key words: tea plant, spermidine, salt stress, stomatal aperture, photosynthetic characteristics

CLC Number: