Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (2): 378-394.doi: 10.16420/j.issn.0513-353x.2020-0946
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YANG Ni1, WAN Qiwen1, LI Yimin1, HAN Miaohua1, TENG Ruimin1, LIU Jiexia2, ZHUANG Jing1,*()
Received:
2021-05-17
Revised:
2021-10-23
Online:
2022-02-25
Published:
2022-02-28
Contact:
ZHUANG Jing
E-mail:zhuangjing@njau.edu.cn
CLC Number:
YANG Ni, WAN Qiwen, LI Yimin, HAN Miaohua, TENG Ruimin, LIU Jiexia, ZHUANG Jing. Effects of Exogenous Spermidine on Photosynthetic Characteristics and Gene Expression of Key Enzymes Under Salt Stress in Tea Plant[J]. Acta Horticulturae Sinica, 2022, 49(2): 378-394.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2020-0946
基因 Gene | 名称 Name | 正向引物(5′-3′) Forward primer sequence | 反向引物(5′-3′) Reverse primer sequence |
---|---|---|---|
CsACT7 | 肌动蛋白 Actin | TTGGATTCTGGGGATGGTGTTAGC | AGCAAGTTTCTCTTTCACATCACGG |
CsADC | 精氨酸脱酸酶 Arginine decarboxylase | TCAAGGAAGGGTCTTTGGGTA | GGTATGTGCGAATGGGGTCT |
CsCHLM | Mg-原卟啉 Ⅸ 甲基转移酶 Magnesium protoporphyrin Ⅸ methyltransferase | CTCTATTGCCTCATTCCTC | ATTTAGTGTTTGGGTTGGT |
CsCRD1 | Mg-原卟啉 Ⅸ 单甲基酯环化酶 Magnesium protoporphyrin Ⅸ monomethyl ester cyclase | CAATGACTGGAAGGCTAA | ATTCTTTGGTGTTGAGGC |
CsFBPase | 果糖-1,6-二磷酸酶 Furetose-1,6-bisphosphate phosphatase | GCAGATTGCTTCGTTGGTTC | TGCTATTATCCCTGTCCTCCC |
CsHEMA1 | 谷氨酸-tRNA还原酶 Glutamyl-tRNA reductase | ATTCGTTGCGAGATTGTT | GCTGCTCCTTTCCTTTGT |
CsHEMC | 胆色素原脱氨酶 Porphobilinogen deaminase | TGACCGCCATTCTTTCTA | GCTAATCTTGTTTCCTCGT |
CsHEMD | 尿卟啉原 Ⅲ 合成酶 Uroporphyrinogen Ⅲ synthase | TGTCTGGGCTGTCTTCGA | CAAATCAGGCAACCGTGT |
CsHEME2 | 尿卟啉原 Ⅲ 脱羧酶 Uroporphyrinogen Ⅲ decarboxylase | ACATTCGCTTCTGTTCCC | TTTCTACTTCCAGCCCTC |
CsHEMG2 | 原卟啉原氧化酶 Protoporphyrinogen oxidase | TCTGTGGAAGAAACGGAACT | CCGCAACGAAAGGGTCAA |
CsODC | 鸟氨酸脱羧酶 Ornithine decarboxylase | TTCCAAACATGGGTGCGTAT | TGTGAGGTGGGTCGTAATCG |
CsPRK | 核酮糖-5-磷酸激酶 Ribulose5-phosphate kinase | AGTATTGGAGCCCGAAAGCC | CAAACAGGTAAACTGGGGTGAA |
CsPPOX | 原卟啉原氧化酶 Protoporphyrinogen oxidase | GAAGCAGTTGACCGTGAC | AAAGCCCTCTGAACCCAC |
CsRbcL | 核酮糖-1,5-二磷酸羧化/加氧酶大亚基 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | TGGCTGGAGATGGGACGA | CCTCTGGTAATCAGAACAGGGTT |
CsSAMDC | S-腺苷甲硫氨酸脱羧酶 S-adenosylmethionine decarboxylase | TACTCTGCTTCTGCTGAATCGA | GCCTTCTTCCTGTCCAAACC |
CsSPDS | 亚精胺合成酶 Spermidine synthases | AACCCAATAGACGCCGATGA | CGCAAAAGATGGCAAACAGA |
CsSAMS | S-腺苷甲硫氨酸合成酶 S-adenosylmethionine synthases | CGATGAGACCGTGACAAATGA | AGGGTTGAGGTGGAAGATGG |
CsTK | 转酮醇酶 Transketolase | TGCCCAATGTTTTGATGCTACG | ATCCACCCTTTTCCACTCCCTC |
Table 1 Quantitative RT-PCR primer sequence
基因 Gene | 名称 Name | 正向引物(5′-3′) Forward primer sequence | 反向引物(5′-3′) Reverse primer sequence |
---|---|---|---|
CsACT7 | 肌动蛋白 Actin | TTGGATTCTGGGGATGGTGTTAGC | AGCAAGTTTCTCTTTCACATCACGG |
CsADC | 精氨酸脱酸酶 Arginine decarboxylase | TCAAGGAAGGGTCTTTGGGTA | GGTATGTGCGAATGGGGTCT |
CsCHLM | Mg-原卟啉 Ⅸ 甲基转移酶 Magnesium protoporphyrin Ⅸ methyltransferase | CTCTATTGCCTCATTCCTC | ATTTAGTGTTTGGGTTGGT |
CsCRD1 | Mg-原卟啉 Ⅸ 单甲基酯环化酶 Magnesium protoporphyrin Ⅸ monomethyl ester cyclase | CAATGACTGGAAGGCTAA | ATTCTTTGGTGTTGAGGC |
CsFBPase | 果糖-1,6-二磷酸酶 Furetose-1,6-bisphosphate phosphatase | GCAGATTGCTTCGTTGGTTC | TGCTATTATCCCTGTCCTCCC |
CsHEMA1 | 谷氨酸-tRNA还原酶 Glutamyl-tRNA reductase | ATTCGTTGCGAGATTGTT | GCTGCTCCTTTCCTTTGT |
CsHEMC | 胆色素原脱氨酶 Porphobilinogen deaminase | TGACCGCCATTCTTTCTA | GCTAATCTTGTTTCCTCGT |
CsHEMD | 尿卟啉原 Ⅲ 合成酶 Uroporphyrinogen Ⅲ synthase | TGTCTGGGCTGTCTTCGA | CAAATCAGGCAACCGTGT |
CsHEME2 | 尿卟啉原 Ⅲ 脱羧酶 Uroporphyrinogen Ⅲ decarboxylase | ACATTCGCTTCTGTTCCC | TTTCTACTTCCAGCCCTC |
CsHEMG2 | 原卟啉原氧化酶 Protoporphyrinogen oxidase | TCTGTGGAAGAAACGGAACT | CCGCAACGAAAGGGTCAA |
CsODC | 鸟氨酸脱羧酶 Ornithine decarboxylase | TTCCAAACATGGGTGCGTAT | TGTGAGGTGGGTCGTAATCG |
CsPRK | 核酮糖-5-磷酸激酶 Ribulose5-phosphate kinase | AGTATTGGAGCCCGAAAGCC | CAAACAGGTAAACTGGGGTGAA |
CsPPOX | 原卟啉原氧化酶 Protoporphyrinogen oxidase | GAAGCAGTTGACCGTGAC | AAAGCCCTCTGAACCCAC |
CsRbcL | 核酮糖-1,5-二磷酸羧化/加氧酶大亚基 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit | TGGCTGGAGATGGGACGA | CCTCTGGTAATCAGAACAGGGTT |
CsSAMDC | S-腺苷甲硫氨酸脱羧酶 S-adenosylmethionine decarboxylase | TACTCTGCTTCTGCTGAATCGA | GCCTTCTTCCTGTCCAAACC |
CsSPDS | 亚精胺合成酶 Spermidine synthases | AACCCAATAGACGCCGATGA | CGCAAAAGATGGCAAACAGA |
CsSAMS | S-腺苷甲硫氨酸合成酶 S-adenosylmethionine synthases | CGATGAGACCGTGACAAATGA | AGGGTTGAGGTGGAAGATGG |
CsTK | 转酮醇酶 Transketolase | TGCCCAATGTTTTGATGCTACG | ATCCACCCTTTTCCACTCCCTC |
处理后天数/d Days after treatment | 处理/(mmol · L-1) Treatment | 气孔横径/μm Stomatal transverse diameter | 气孔纵径/μm Stomatal longitudinal diameter | 气孔开度/μm2 Stomatal aperture | |
---|---|---|---|---|---|
NaCl | Spd | ||||
1 | 0(对照Control) | 0 | 8.75 ± 0.13 lm | 17.81 ± 0.71 def | 122.54 ± 6.67 jk |
0 | 0.5 | 11.88 ± 0.16 abcde | 20.21 ± 0.41 abcd | 188.49 ± 6.36 b | |
0 | 1.0 | 9.60 ± 0.29 ijklm | 18.24 ± 0.73 bcdef | 137.16 ± 2.38 hi | |
0 | 1.5 | 12.51 ± 0.22 a | 18.79 ± 0.20 abcde | 184.48 ± 1.27 bc | |
200 | 0 | 10.44 ± 0.31 efghijk | 18.83 ± 0.46 abcde | 154.03 ± 1.55 fg | |
200 | 0.5 | 8.69 ± 0.19 lm | 17.15 ± 0.29 ef | 116.85 ± 1.28 k | |
200 | 1.0 | 10.87 ± 0.32 cdefghi | 16.11 ± 0.21 f | 137.47 ± 4.17 hi | |
200 | 1.5 | 9.88 ± 0.29 hijkl | 18.85 ± 0.74 abcde | 145.80 ± 2.59 gh | |
3 | 0(对照Control) | 0 | 10.39 ± 0.01 efghijk | 17.61 ± 0.38 def | 143.64 ± 3.33 gh |
0 | 0.5 | 10.05 ± 0.09 ghijkl | 19.25 ± 0.45 abcde | 151.90 ± 2.27 g | |
0 | 1.0 | 11.10 ± 0.27 abcdefgh | 19.64 ± 0.85 abcde | 171.00 ± 3.29 de | |
0 | 1.5 | 12.32 ± 0.62 abc | 19.54 ± 0.60 abcde | 188.59 ± 6.56 b | |
200 | 0 | 11.74 ± 0.55 abcde | 19.16 ± 0.99 abcde | 175.68 ± 1.93 bcde | |
200 | 0.5 | 9.11 ± 0.35 klm | 21.08 ± 0.48 a | 150.63 ± 2.34 g | |
200 | 1.0 | 9.09 ± 0.15 klm | 17.53 ± 0.48 def | 125.04 ± 1.40 ijk | |
200 | 1.5 | 9.81 ± 0.66 hijkl | 17.91 ± 1.08 cdef | 136.88 ± 1.09 hi | |
5 | 0(对照Control) | 0 | 10.92 ± 0.41 bcdefghi | 20.09 ± 1.14 abcd | 171.96 ± 8.84 cde |
0 | 0.5 | 11.16 ± 0.68 abcdefgh | 20.54 ± 0.47 abc | 179.77 ± 6.74 bcd | |
0 | 1.0 | 10.64 ± 0.53 defghij | 20.17 ± 0.70 abcd | 167.52 ± 2.31 de | |
0 | 1.5 | 12.39 ± 0.25 ab | 21.29 ± 0.35 a | 206.93 ± 1.20 a | |
200 | 0 | 10.98 ± 0.32 bcdefghi | 19.89 ± 0.59 abcd | 171.24 ± 0.81 de | |
200 | 0.5 | 10.72 ± 0.56 defghij | 20.99 ± 1.62 a | 175.85 ± 4.47 bcde | |
200 | 1.0 | 11.55 ± 0.16 abcdef | 19.15 ± 0.17 abcde | 173.63 ± 4.02 cde | |
200 | 1.5 | 11.53 ± 0.63 abcdefg | 19.09 ± 1.07 abcde | 171.28 ± 3.86 de | |
9 | 0(对照Control) | 0 | 11.50 ± 0.21 abcdefg | 18.25 ± 0.34 bcdef | 164.74 ± 3.18 ef |
0 | 0.5 | 9.27 ± 0.47 jklm | 17.69 ± 1.03 def | 128.15 ± 5.21 ijk | |
0 | 1.0 | 12.27 ± 0.33 abc | 19.54 ± 0.74 abcde | 187.86 ± 2.88 b | |
0 | 1.5 | 11.79 ± 0.08 abcde | 18.78 ± 0.51 abcde | 173.70 ± 3.57 cde | |
200 | 0 | 8.28 ± 0.41 m | 20.08 ± 0.73 abcd | 129.99 ± 1.68 ij | |
200 | 0.5 | 11.56 ± 0.34 abcdef | 20.72 ± 0.45 ab | 187.98 ± 1.47 b | |
200 | 1.0 | 11.97 ± 0.39 abcd | 19.10 ± 0.59 abcde | 178.85 ± 2.61 bcd | |
200 | 1.5 | 10.13 ± 0.22 fghjkl | 18.94 ± 0.82 abcde | 150.29 ± 3.32 g |
Table 2 Effects of exogenous Spd on stomatal aperture of tea plant leaves under salt stress
处理后天数/d Days after treatment | 处理/(mmol · L-1) Treatment | 气孔横径/μm Stomatal transverse diameter | 气孔纵径/μm Stomatal longitudinal diameter | 气孔开度/μm2 Stomatal aperture | |
---|---|---|---|---|---|
NaCl | Spd | ||||
1 | 0(对照Control) | 0 | 8.75 ± 0.13 lm | 17.81 ± 0.71 def | 122.54 ± 6.67 jk |
0 | 0.5 | 11.88 ± 0.16 abcde | 20.21 ± 0.41 abcd | 188.49 ± 6.36 b | |
0 | 1.0 | 9.60 ± 0.29 ijklm | 18.24 ± 0.73 bcdef | 137.16 ± 2.38 hi | |
0 | 1.5 | 12.51 ± 0.22 a | 18.79 ± 0.20 abcde | 184.48 ± 1.27 bc | |
200 | 0 | 10.44 ± 0.31 efghijk | 18.83 ± 0.46 abcde | 154.03 ± 1.55 fg | |
200 | 0.5 | 8.69 ± 0.19 lm | 17.15 ± 0.29 ef | 116.85 ± 1.28 k | |
200 | 1.0 | 10.87 ± 0.32 cdefghi | 16.11 ± 0.21 f | 137.47 ± 4.17 hi | |
200 | 1.5 | 9.88 ± 0.29 hijkl | 18.85 ± 0.74 abcde | 145.80 ± 2.59 gh | |
3 | 0(对照Control) | 0 | 10.39 ± 0.01 efghijk | 17.61 ± 0.38 def | 143.64 ± 3.33 gh |
0 | 0.5 | 10.05 ± 0.09 ghijkl | 19.25 ± 0.45 abcde | 151.90 ± 2.27 g | |
0 | 1.0 | 11.10 ± 0.27 abcdefgh | 19.64 ± 0.85 abcde | 171.00 ± 3.29 de | |
0 | 1.5 | 12.32 ± 0.62 abc | 19.54 ± 0.60 abcde | 188.59 ± 6.56 b | |
200 | 0 | 11.74 ± 0.55 abcde | 19.16 ± 0.99 abcde | 175.68 ± 1.93 bcde | |
200 | 0.5 | 9.11 ± 0.35 klm | 21.08 ± 0.48 a | 150.63 ± 2.34 g | |
200 | 1.0 | 9.09 ± 0.15 klm | 17.53 ± 0.48 def | 125.04 ± 1.40 ijk | |
200 | 1.5 | 9.81 ± 0.66 hijkl | 17.91 ± 1.08 cdef | 136.88 ± 1.09 hi | |
5 | 0(对照Control) | 0 | 10.92 ± 0.41 bcdefghi | 20.09 ± 1.14 abcd | 171.96 ± 8.84 cde |
0 | 0.5 | 11.16 ± 0.68 abcdefgh | 20.54 ± 0.47 abc | 179.77 ± 6.74 bcd | |
0 | 1.0 | 10.64 ± 0.53 defghij | 20.17 ± 0.70 abcd | 167.52 ± 2.31 de | |
0 | 1.5 | 12.39 ± 0.25 ab | 21.29 ± 0.35 a | 206.93 ± 1.20 a | |
200 | 0 | 10.98 ± 0.32 bcdefghi | 19.89 ± 0.59 abcd | 171.24 ± 0.81 de | |
200 | 0.5 | 10.72 ± 0.56 defghij | 20.99 ± 1.62 a | 175.85 ± 4.47 bcde | |
200 | 1.0 | 11.55 ± 0.16 abcdef | 19.15 ± 0.17 abcde | 173.63 ± 4.02 cde | |
200 | 1.5 | 11.53 ± 0.63 abcdefg | 19.09 ± 1.07 abcde | 171.28 ± 3.86 de | |
9 | 0(对照Control) | 0 | 11.50 ± 0.21 abcdefg | 18.25 ± 0.34 bcdef | 164.74 ± 3.18 ef |
0 | 0.5 | 9.27 ± 0.47 jklm | 17.69 ± 1.03 def | 128.15 ± 5.21 ijk | |
0 | 1.0 | 12.27 ± 0.33 abc | 19.54 ± 0.74 abcde | 187.86 ± 2.88 b | |
0 | 1.5 | 11.79 ± 0.08 abcde | 18.78 ± 0.51 abcde | 173.70 ± 3.57 cde | |
200 | 0 | 8.28 ± 0.41 m | 20.08 ± 0.73 abcd | 129.99 ± 1.68 ij | |
200 | 0.5 | 11.56 ± 0.34 abcdef | 20.72 ± 0.45 ab | 187.98 ± 1.47 b | |
200 | 1.0 | 11.97 ± 0.39 abcd | 19.10 ± 0.59 abcde | 178.85 ± 2.61 bcd | |
200 | 1.5 | 10.13 ± 0.22 fghjkl | 18.94 ± 0.82 abcde | 150.29 ± 3.32 g |
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