Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 389-396.doi: 10.16420/j.issn.0513-353x.2020-0318

• New Technology and New Mathod • Previous Articles     Next Articles

Multiplex PCR Detection for Late Blight Resistant Genes R8,RB and Virus Resistant Genes Rx1,Ryadg in Potato

LIU Cheng1,*, WANG Shiyao1,*, SHI Weiling1, SONG Yuhao1, JIANG Rui1, ZHAO Yong1, MO Shichun2, LÜ Dianqiu1, WANG Jichun1, LIU Xun1,**()   

  1. 1Chongqing Key Laboratory of Biology and Genetic Improvement in Tuber and Root Crops,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China
    2Chongqing Wuxi Agricultural and Rural Committee,Chongqing 405899,China
  • Received:2020-04-26 Revised:2020-06-01 Online:2021-02-25 Published:2021-03-09
  • Contact: LIU Xun


Based on defined RB and R8 genes for late blight resistance in 218 germplasm resources,Rx1 and Ryadg for potato virus X(PVX)and potato virus Y(PVY)extreme resistance were screened in the present study,respectively. The mixed DNA from two screened potato genotypes containing Rx1,RB and Ryadg,R8 was used as templates. The multiplex PCR system was developed with the reported markers for Rx1 and Ryadg and designed markers for RB and R8. In addition,the amplification of the PCR reaction system was monitored by the potato GBSS gene markers. The crucial factors of multiplex PCR system including extension temperature and primers concentration were optimized. Five specific fragments were simultaneously amplified in one PCR reaction. This detection method was used to detect these R genes from advanced breeding lines. The results revealed that four breeding lines containing four above mentioned R genes were screened. In conclusion,the established multiplex PCR detection system will be used for molecular marker-assisted selection,providing a simple and efficient technical method for late blight and viral diseases multi-R genes aggregation breeding,and new resources for the new varieties selection with four R genes in potato.

Key words: potato, late blight, potato virus X, potato virus Y, multiplex PCR

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