Identification and Biological Characteristics of a Novel Pathogen Fusarium incarnatum Causing Muskmelon Fruit Fusarium Rot

doi:10.16420/j.issn.0513-353x.2018-0820

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (3): 529-539.doi: 10.16420/j.issn.0513-353x.2018-0820

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Identification and Biological Characteristics of a Novel Pathogen Fusarium incarnatum Causing Muskmelon Fruit Fusarium Rot

WANG Yan1，WANG Chunwei1,*，WANG Lin2，LI Zhiyuan1，ZHANG Xiqian1，LIU Zheng1，CHEN Ermao1，WANG Meiqin1，ZHANG Zuogang1，and WANG Jianming1,*

（1College of Agriculture，Shanxi Agricultural University，Taigu，Shanxi 030801，China；2Yuncheng Sub-center，Shanxi Entry-Exit Inspection and Quarantine Bureau Technology Center，Yuncheng，Shanxi 044600，China）

Online:2019-03-25 Published:2019-03-25

Abstract

Abstract: A novel disease of suspected Fusarium fruit rot was observed on muskmelon in Taigu County，Shanxi Province. The isolate was obtained from diseased sample by conventional tissue isolation method. The pathogenicity was confirmed by Koch’s postulates. The pathogen was identified as Fusarium incarnatum based on morphological characteristics and the sequence analysis of rDNA-ITS，EF-1α，histone 3 and mtSSU. The results of biological characteristics showed that PSA medium was most conducive to mycelium growth and sporulation；the ideal temperature conditions for mycelium growth and sporulation were in the range of 20–35 ℃ and 20–25 ℃，respectively；the suitable carbon sources for mycelium growth were CMC-Na，starch and xylose，the optimal carbon source for sporulation was lactose. The pathogen could grow fastly on the medium with kinds of nitrogen sources，the optimal nitrogen source for sporulation was yeast powder. The optimal pH for mycelium growth was 8. The full illumination and full darkness was suitable for mycelium growth. The sporulation under pH and light conditions had not significant difference. The mycelial lethal temperature was at 61 ℃ for 10 min，and the spore lethal temperature was at 56 ℃ for 10 min.

Key words: muskmelon, fruit rot, Fusarium incarnatum, identification, biological characteristics

CLC Number:

S 652

WANG Yan1，WANG Chunwei1,*，WANG Lin2，LI Zhiyuan1，ZHANG Xiqian1，LIU Zheng1，CHEN Ermao1，WANG Meiqin1，ZHANG Zuogang1，and WANG Jianming1,*. Identification and Biological Characteristics of a Novel Pathogen Fusarium incarnatum Causing Muskmelon Fruit Fusarium Rot[J]. ACTA HORTICULTURAE SINICA, 2019, 46(3): 529-539.

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Identification and Biological Characteristics of a Novel Pathogen Fusarium incarnatum Causing Muskmelon Fruit Fusarium Rot

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