Abstract: Forty-two leaf color mutants of Chinese cabbage obtained through EMS seeds mutagenesis were used as materials in this study. According to leaf color and leaf chlorophyll content at generative growth mutations were suggested to be divided into 9 types：Dark green，gray-green，green，light green，white-green，light white-green，yellow-green，light yellow-green and yellow. By detecting the nucleotide variation of the gene HCF164 related to chlorophyll fluorescence using HRM technology and by measuring chlorophyll fluorescence characteristics，we identified one yellow-green leaf color mutant A29 with high photosynthesis efficiency，one yellow-green leaf color mutant A35 with photosynthetic structure damages，one light green mutant A21 with photosynthetic electron transport obstruction. Through identifying other 7 leaf-color-related genes by HRM，mutation of chlorophyll-related genes ATRCCR，CLH2 and PORA could be the main reason resulted in 18 leaf color mutants，mutation of yellow-leaf- specific genes was also affected the variation of leaf color.

Key words: Chinese cabbage, EMS mutation, leaf color, HRM, chlorophyll fluorescence