Abstract: According to the gene sequence differences，primers were designed for 4 disease resistance genes in tomato，Tm-2，Pto，Sw-5 and Ve1. After checking of primer specificity and aligning of the clone sequences of the PCR products，the HRM（High resolution melting）technology was used for polymorphism detection. In this study，5 SNPs were developed，all of which could distinguish the materials with different genotypes. What’s more，the genotype was completely consistent with the known gene. So they can be used as functional markers in tomato disease resistance breeding.

Key words: tomato, disease resistance gene, functional marker, HRM