Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (8): 1475-1486.

• Vegetables • Previous Articles Next Articles

Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）

XU Wen-ling1,2，WANG Shu-fen2，WANG Cui-hua2，LIU Xian-xian2，FU Wei-min2，HE Qi-wei2,*，and ZHAO Shuang-yi3,*

（1College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China；2Institute of Vegetable Research of Shandong Academy of Agricultural Science，Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province，National Improvement Center for Vegetable，Shandong Branch，Ji’nan 250100，China；3School of Life Science of Shandong University，Ji’nan 250100，China）

Received:2013-04-11 Online:2013-08-25 Published:2013-08-25

Abstract

Abstract: To further reveal the molecular mechanism of pol CMS fertility-restorer genes，digital gene expression profiling technology was used to choose the F₂ segregating population hybrid of sterile lines and restorer lines. By using the F₂ lines，differential expressions of Chinese cabbage pol CMS fertility- restorer genes were analyzed and real-time fluorescence quantitative PCR was used to validate some genes. There were 2 826 differentially expressed genes，441 of which were up-regulated，and 2 835，down-regulated. GO functional annotations showed that many differentially expressed genes were significantly clustered in cytoplasm，organelle，and macromolecular complex etc. Organelles were mainly mitochondria，chloroplasts and plastid etc. These genes showed exonuclease activities and were. involved in the biological processes of pollen wall formation and assembly. Metabolic pathways significantly related to pol CMS were mainly the pathways of ribosomes，glucose and amino acid metabolism，peroxidase，nucleotide excision and repair，and RNA degradation. The gene expression profile and RT-PCR results indicate that restorer genes regulate fertility restoration by down-regulation. Further，four differentially expressed genes were shown to be closely related to fertility restoration were closely related to fertility restoration.

Key words: Chinese cabbage, pol CMS, fertility restorer gene, differentially expression, real-time qPCR

XU Wen-ling1,2，WANG Shu-fen2，WANG Cui-hua2，LIU Xian-xian2，FU Wei-min2，HE Qi-wei2,*，and ZHAO Shuang-yi3,*. Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）[J]. ACTA HORTICULTURAE SINICA, 2013, 40(8): 1475-1486.

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Gene Expression Profiling Analysis of pol CMS Fertility-restorer Genes in Chinese Cabbage（Brassica rapa L. ssp. pekinensis）

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