Abstract: In this study，a population of F2 generation including 180 individual plants was derived from
the interspecific cross between the female line Capsicum annuum B9431 and the male line C. frutescens H108.
The SRAP，SSR，ISSR markers and morphological marker were used in constructing a genetic map. A
genetic linkage map containing 14 linkage groups（LGs）was constructed using 264 SRAP，32 SSR and 2
ISSR. The S gene controlling the soft flesh and deciduous fruit was located at LG8. The map covered 1 023.45
cM with an average marker interval of 3.42 cM. Number of markers included in linkage groups varied
from 2 to 44，mean marker interval distance from 2.38 cM to 12.90 cM and the length of linkage groups
from 13.84 cM to 129.93 cM individually. Furthermore，the links between the map published by
Minamiyama et al. and ours were established by the public microsatellites（SSR markers）.

Key words: pepper, interspecific genetic map, SRAP, SSR, ISSR