Characterization of the Chinese Cabbage hau Cytoplasmic Male-sterile Line and Sequence Analysis of the Fertility-related Gene

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (3): 469-476.

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Characterization of the Chinese Cabbage hau Cytoplasmic Male-sterile Line and Sequence Analysis of the Fertility-related Gene

SHI Zhan，WAN Zheng-jie^*，XU Yue-jin，LI Xue-hong，and ZOU Rui-chang

College of Horticulture and Forestry，Huazhong Agricultural University，Education Ministry Key Laboratory of Horticultural Plant Biology，Huazhong Agricultural University，Wuhan 430070，China

Received:2011-12-19 Revised:2012-02-21 Online:2012-03-25 Published:2012-03-25
Contact: WAN Zheng-jie E-mail:wanzj@mail.hzau.edu.cn

Abstract

Abstract: The male-sterility of the Chinese cabbage（Brassica campestris L. ssp. pekinensis）line 6w-9605A，a cytoplasmic male-sterile line，was studied in this research. Chromosome squashing revealed that the chromosome numbers in the F₁and BC₆ of 6w-9605A were 28 and 20，respectively，and morphologic observation in the BC₆of 6w-9605A showed that the anthers of this line were degenerated and completely abortive. Further，primers were designed based on the conserved sequence of known cytoplasmic male sterility genes in the Brassica species，and a new open reading frame（ORF）of 867 base pairs was amplified from 6w-9605A. Sequence analysis showed that this ORF located downstream from the mitochondrial gene apt6 and was predicted as orf288. Sequence alignment demonstrated that the ORF is different from the sterile genes of pol CMS and ogu CMS，and it may cause the sterility of 6w-9605A.

Key words: Chinese cabbage, male-sterility, hau cytoplasm male-sterile line, infertility genes

SHI Zhan, WAN Zheng-Jie, XU Yue-Jin, LI Xue-Hong, ZOU Rui-Chang. Characterization of the Chinese Cabbage hau Cytoplasmic Male-sterile Line and Sequence Analysis of the Fertility-related Gene[J]. ACTA HORTICULTURAE SINICA, 2012, 39(3): 469-476.

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Characterization of the Chinese Cabbage hau Cytoplasmic Male-sterile Line and Sequence Analysis of the Fertility-related Gene

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