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ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (6): 1180-1184.

• Research Notes • Previous Articles     Next Articles

Identification and PCR Detection of Phytophthora Blight of Lily

YANG Xiu-mei1,QU Su-ping1,WANG Li-hua1,LIU Xiao-xia2,PENG Lü-chun1,and WANG Ji-hua1,*   

  1. (1Flower Research Institute,Yunnan Academy of Agriculture Sciences,Yunnan Key Laboratory for Flower Breeding,Kunming 650205,China;2Biochemical College of Jiaxing University,Jiaxing,Zhejiang 314001,China)   
  • Received:2011-01-20 Revised:2011-05-03 Online:2011-06-25 Published:2011-06-25
  • Contact: WANG Ji-hua

Abstract: The pathogen causing lily Phytophthora blight was isolated from diseased plants with typical symptoms on basal stems. The fungi was identified as Phytophthora nicotianae according to morphological characteristics,pathogenicity and the sequence of ribosomal DNA-ITS. The sequence of ribosomal DNA-ITS of isolate P1 was showed a sequence identity of 98%–99% with Phytophthora nicotianae published in GenBank. PCR detection using P. nicotianae–specific primers indicated that the pathogen was P. nicotianae. This is the first report of Phytophthora blight of lily in Yunnan.   

Key words: lily, Phytophthora blight, pathogen identification, PCR detection

CLC Number: