Identification of Self-incompatibility SFB Genes for Apricot Cultivars in Xinjiang Area

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (4): 666-674.

• Fruit Trees • Previous Articles Next Articles

Identification of Self-incompatibility SFB Genes for Apricot Cultivars in Xinjiang Area

LIU Yue-xia，FENG Jian-rong*，WANG Da-jiang，FAN Xin-min，and JIANG Xin

College of Agriculture，Shihezi University，Shihezi，Xinjiang 832003，China

Received:2010-09-17 Revised:2011-03-14 Online:2011-04-25 Published:2011-04-25
Contact: LIU Yue-xia

Abstract

Abstract: In order to identify SFB gene associated with self-incompatibility of apricot cultivars in Xinjiang area systematically，five pairs of primers designed according to the conserved sequences of Rosaceae SFB were used for specific PCR amplification of 25 mainly apricot cultivars in Xinjiang. The specific fragments amplified successfully were cloned and sequenced. The results showed：The primer combinationⅡ-1、Ⅳ-2，1-Ⅰ、1-Ⅱ was the best optional；Five different size fragments and 14 different genes were cloned from 18 apricot cultivars. Searches for homology with our sequences publicly available DNA databases revealed 2 SFB gene fragments（SFB-36，SFB-42）had been deposited in the GenBank （Accession No：EF062342）as SFB-13，and the other 12 SFB gene fragments were novel SFB genes，temporarily named as：SFB-30，SFB-31，SFB-32，SFB-33，SFB-34，SFB-35，SFB-37，SFB-38，SFB-39，SFB-40，SFB-41 and SFB-43. Comparison of amino acid sequences of this 14 SFB gene fragments in apricot revealed three conserved regions and two high variability regions. Sixty-two SFB genes in Rosaceae 　　

Key words: apricot, self-incompatibility, SFB gene

LIU Yue-xia;FENG Jian-rong;WANG Da-jiang;FAN Xin-min;and JIANG Xin . Identification of Self-incompatibility SFB Genes for Apricot Cultivars in Xinjiang Area [J]. ACTA HORTICULTURAE SINICA, 2011, 38(4): 666-674.

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Identification of Self-incompatibility SFB Genes for Apricot Cultivars in Xinjiang Area

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