Cloning and Recombinant Protein Expression of ACC Synthase Genes in Cut Roses（Rosa hybrida）

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (7): 1132-1138.

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Cloning and Recombinant Protein Expression of ACC Synthase Genes in Cut Roses（Rosa hybrida）

TAN Yuan-jun1，XUE Jing-qi2，TONG Zheng1，MA Nan1，and GAO Jun-ping1,*

（1Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University，Beijing 100193，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China）

Received:2010-06-15 Revised:2010-06-22 Online:2010-07-25 Published:2010-07-25
Contact: GAO Jun-ping

Abstract

Abstract: Our previous work showed that the expression of ACC synthase genes was contributed to the effects of ethylene on hastening flower opening. In the present work，we isolated the wound-inducible and ethylene up-regulated ACS genes，namely Rh-ACS1 and Rh-ACS3，from cut rose petals. The full length of Rh-ACS1 is 2 132 bp，contains a 1 476 bp ORF which encodes a putative ACS protein with 491 aa，while the entire Rh-ACS3 is 1 734 bp in length，and contains a 1 545 bp ORF which encodes a putative ACS protein with 514 aa. The ORFs of Rh-ACS1 and Rh-ACS3 were inserted into pGEX-4T-1 and pET-30a to express the recombinant proteins in E.coli，respectively. The expression of predicted 70 kD and 60 kD recombinant proteins were induced by isopropyl β-D-thiogalacto-pyranoside（IPTG）at a final concentration of 0.6 mmol · L-1 for 3 h at 37 ℃.

Key words: cut rose, ACS, gene cloning, procaryotic expression

CLC Number:

S 685.12

TAN Yuan-jun;XUE Jing-qi;TONG Zheng;MA Nan;and GAO Jun-ping;. Cloning and Recombinant Protein Expression of ACC Synthase Genes in Cut Roses（Rosa hybrida） [J]. ACTA HORTICULTURAE SINICA, 2010, 37(7): 1132-1138.

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Cloning and Recombinant Protein Expression of ACC Synthase Genes in Cut Roses（Rosa hybrida）

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