Abstract: Different levels of Na2SeO3（0，50，100，200 and 400 μg · mL-1） and（CH3COO）2 Zn · 2H2O（0，0.2%，0.4%，0.8% and 1.2%） was applied by foliar spraying to determine the effects of Se，Zn and the their interaction on the subcellular distribution of Selenium in spring tea leaves. The result showed that：（1）The Se content in subcellular part of the cell wall，membrane and organelle part，soluble fraction compared with the control increased in varying degrees in different treatments of Se. The Se was mainly distributed in membranes and organelles.（2）The increased effects of Se content in different subcellular components was obvious in lower concentration（0.2%–0.4%）of zinc treatment，but the Se content of the different subcellular components was significantly lower and the Se assimilation was restrained when the zinc concentration increased to 0.8%–1.2%. （3）The content and the distributing proportion of selenium in membrane and organelle part were the highest，and the second was cell wall，the third was soluble fraction. Combined applying middle concentration of Zn（0.4%–0.8%）and Se（100–400 μg · mL-1） increased obviously the Se content in different subcellular component，but combined applying high-concentration of Se and different Zn concentrations had no significant effects on the Se accumulation. The Se in cell wall and soluble fraction was transferred to membrane and organelle part obviously when combined applying lower Se concentration（50–100 μg · mL-1）and different Zn concentrations. The Se of subcellular was transferred mainly in the between the membrane and organelle part and soluble fraction when combined applying higher Se concentration and different Zn concentrations. In general，whether single Se and Zn treatment or Se-Ze combined applying treatment，it could significantly affect the Se distribution in tea subcellular cells. The Se was mainly distributed in membranes and organelles by Se and Se-Zn interaction，and accumulated in cell wall by single Zn treatment.

Key words: tea, Se-Zn interaction, subcellular