Components related to aroma, sugar, acid were assayed in mature fruits of two wild pentaploid strawberry accessions native to China, Hei 1 and Hei 6, by HS-SPME, GC–MS and HPLC. The results showed that the accessions contained 92 aromatic components divided into 11 categories, which included 22 components（5 categories）with relative contents higher than 1%. The predominant aromatic components were acetic acid ethyl ester, butanoic acid ethyl ester, butanoic acid 3-methyl ethyl ester, hexanoic acid ethyl ester, myrtenyl acetate,2-pentanone, 2-undecanone, ethanol，2-heptanol, 1-hexanol, （E）-2-hexen-1-ol, hexanal,（E）-2-hexenal, 2,5-dimethyl-4-methoxy-3（2H）-furanone. Butanoic acid 3-methyl ethyl ester was the unique component in the two wild pentaploid accessions in comparison with Hei 10 （Fragaria orientalis，4x） and Ji 12 （F. mandschurica，2x）, and 2, 5-dimethyl-4-methoxy-3（2H）-furanone, 2-pentanone, 2-heptanol, 1-hexanol, and （E）-2-hexen-1-ol were more abundant in the pentaploids. Fructose was the major sugar, followed by glucose, while the least was sucrose. Remarkable difference in sorbitol content was observed between the two accessions. Malic acid predominated in the two wild pentaploid accessions, more than 86% of the total acids.