Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (1): 116-130.doi: 10.16420/j.issn.0513-353x.2021-0834

• Research Papers • Previous Articles     Next Articles

Analysis of Petal Volatile Components Among Different Hemerocallis Cultivars Based on HS-SPME-GC-MS

QIANG Wenyan1, MENG Qingran2, ZHANG Zhiguo1, GAO Wenjie1,*()   

  1. 1Department of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China
    2Department of Flavor and Fragrance Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China
  • Received:2022-07-13 Revised:2022-12-31 Online:2023-01-25 Published:2023-01-18
  • Contact: *(


Based on HS-SPME combined with GC-MS technology,the optimum extraction temperature and time of the volatile components from Hemerocallis‘Shaman’were firstly determined. Then,the nine H. fulva cultivars of‘Free Wheelin’’‘She’s Got the Look’‘Forty Second Street’‘Grape Velvet’‘Lilly and Petals’‘Unlock the Stars’‘Web of Illusion’‘Fire on the Mountain’and‘Shaman’in the blooming stage were analyzed by GC-MS. In addition,GC-MS-O and OAVs were used to detect key aroma components in different cultivars. The results showed that the best extraction temperature was 80 ℃,and the best extraction time was 40 min. Based on this condition,a total of 197 volatile components were detected,including alcohols,terpenes,aldehydes,nitriles,phenols,esters,alkanes,furans,and a small amount of other substances. By combining peak area normalization method with GC-MS-O,27 volatile components were identified as aroma components. Based on the principal component analysis(PCA)and hierarchical cluster analysis(HCA)of 27 main aroma components,(Z)-β-ocimene and Nonanal were identified as the main aroma components in Hemerocallis. Also,it was preliminarily concluded trans-nerolidol,linalool,phenethyl alcohol,indole and estragole etc. may be responsible for the various aroma of different cultivars of H. fulva. Finally,nine samples of H. fulva were divided into four groups,one group was‘Free Wheelin’’and‘She’s Got the Look’,which did not have benzyl nitrile,phenylethyl alcohol and benzeneacetaldehyde,showingmainly woody,herbal,and floral aromas;the other group was‘Grape Velvet’‘Lilly and Petals’‘Unlock the Stars’‘Web of Illusion’and‘Fire on the Mountain’,mainly composed of phenylethyl alcohol,benzaldehyde,geranyl acetone,3-furan methanol and other substances to form floral and fruity aroma characteristics. Moreover,‘Forty Second Street’and‘Shaman’each form a separate group,containing a variety of unique substances and revealing the distinctive aroma characteristics.

Key words: Hemerocallis fulva, volatile components, HS-SPME-GC-MS, pretreatment, aroma

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