Abstract: The spore in vitro culture and routine propagation of Drynaria roosii were studied respectively. Tests were conducted to evaluate the effects of various sterilization procedures, medium and light intensity on spores of Drynaria roosii. The result exhibits that the spores germinated within 6-7 days after inoculated in the medium, and sporophytes began to form in 100 days after inoculated. The maximum germination ratio was 49% obtained in Knop’s medium. The low salt concentration of MS basal medium was helpful to spore germination, but inhibited the development of gametophytes. Spores can germinate in dark but can’t develop to prothallial plate. Spores surface sterilized for 5.5 min with 0.5%NaClO and inoculated in 1/2MS medium, were appropriate for Drynaria roosii cultivation with 40μmol·m^-2·s^-1 light intensity.

Key words: Drynaria roosii Nakaike, spore germination, in vitro culture