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ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (4): 583-586.

• 研究简报 • Previous Articles     Next Articles

Studies on Isolated Microspore Culture of the Allotriploid Hybrids BetweenCabbage and Chinese Cabbage

ZHANG Wei-wei, SHEN Shu-xing*, WANG Yan-hua,CHEN Xue-ping, XUAN Shu-xin, LUO Shuang-xia,and LI Xiao-feng   

  1. (College of Horticulture, Agricultural University of Hebei, B aoding, Hebei 071001, China)
  • Received:2008-10-17 Revised:2009-03-24 Online:2009-04-25 Published:2009-04-25
  • Contact: SHEN Shu-xing

Abstract: Microspore culture of 8 allotriploid hybrids between diploid Chinese cabbage (AA, 2n=2x=20) and autotetrap loid cabbage (CCCC, 2n=4x=36) was performed in order to create the alien addition lines. The results showed that embryo p roduction frequency of the allotrip loid hybrids (ACC) was lower than
their parents. In liquid medium NLN with 15% sucrose and 0.1 mg·L-1 6-BA, 0.2 mg·L-1 2, 4-D, 0.05mg·L-1 KT, the 8 lines were different, the highest of embryo production was 0.0250 embryos per bud, the average was 0.0100 embryos per bud. The p lant regeneration frequency of embryoids was only 33.3%. The chromosome number of plantlets derived from microspore culture varied from 14 to 27. The morphological characters were diverse among the different plants.

Key words: cabbage, Chinese cabbage, microspore culture, embryo, alien addition lines

CLC Number: