In silico Analysis and Expression Confirmation of the Regulation of FruitColoration by Transcriptional Factor MdMBY1 in Delicious Apple

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (11): 1581-1588.

• Fruit Trees • Previous Articles Next Articles

In silico Analysis and Expression Confirmation of the Regulation of FruitColoration by Transcriptional Factor MdMBY1 in Delicious Apple

ZHANG Hua-lei^1,2;MAO Ke^1,2;LIU Zhi^2,3;XIE Xing-bin^1,2;FENG Xiao-ming^1,2; HAO Yu-jin^1,2*

(¹ State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, China; ²College of Horticulture Science and Engineering, Shandong AgriculturalUniversity, Taipan, Shandong 271018, China; ³ Liaoning Institute of Pomology, Xiongyue, Liaoning 115009, China)

Received:2009-07-03 Revised:2009-09-21 Online:2009-11-25 Published:2009-11-25
Contact: HAO Yu-jin

Abstract

Abstract: In this study, MdMYB1 was cloned from Delicious apple. Itwas found that MdMYB1 protein was localized in nucleus indicated by in silico predication and GFP fusion protein fluorescence observation. In parallel, the promoter regions of MdDFT and MdUFGT were isolated and analyzed in silico. The result showed that both promoters contained cis-elements characterized as MYB binding sites. Furthermore, to examine if MdMYB1 triggers the expression of MdDFR and MdUFGT during fruit coloration, anthocyanin content, expression levels of MdMYB1, MdDFR and MdUFGT were investigated. The results demonstrated that light exposure markedly induced anthocyanin accumulation in fruit peel, along with a rapid initiation of gene expression. Interestingly, three genes were expressed in a highly similar pattern. In one word, our findings suggest that transcription factor MdMYB1 may directly regulate the expression of MdDFR and MdUFGT genes.

Key words: apple, fruit, coloration gene, light induction, expression analysis

CLC Number:

S 661.1

ZHANG Hua-lei;MAO Ke;LIU Zhi;XIE Xing-bin;FENG Xiao-ming;HAO Yu-jin;. In silico Analysis and Expression Confirmation of the Regulation of FruitColoration by Transcriptional Factor MdMBY1 in Delicious Apple[J]. ACTA HORTICULTURAE SINICA, 2009, 36(11): 1581-1588.

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In silico Analysis and Expression Confirmation of the Regulation of FruitColoration by Transcriptional Factor MdMBY1 in Delicious Apple

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