Abstract:

The aim of this study is to obtain transgenic pepper plants resistant to TMV and CMV that could be used in breeding new pepper variety with virus-resistant traits. The primers were designed based on the sequences of PAP in Phytolacca Americana to amplify non-virulent defective PAP gene , PacPAP1 from genomic DNA of leaves in Phytolacca acinosa. The expression vectors was constructed and the PacPAP1 was transferred into pepper (Capsicum annuum L.) by flamingo bill mediated-Agrobacterium. In the transgenic plants , the molecular test were done and resistance assay was performed at 25d following artificial inoculation with TMV or CMV. The results showed that the size of the isolated non-virulent defective PacPAP is 714bp, bearing 99.7% identity with PamPAP from Phytolacca american. The accession numbers of the gene in GenBank is AY603353. The transgenic pepper plants were obtained with PacPAP. The non-transgenic pepper plants were susceptible to virus, no symptoms were observed in the positive transgenic plants. This suggests that transgenic pepper plant resistant to TMV and CMV can be obtained by transferring PacPAP1.

Key words: Phytolacca acinosa, pokeweed antiviral protein gene, genetic transformation, pepper