Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk. of Garlic

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (6): 841-846.

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Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk. of Garlic

ZHANG lin-qing^1,2and CHENG Zhi-hui^1*

(¹CoLLege of Horticulture, Northwest A & F University, Yangling, Shaanxi 712100, China; ²Huaiyin Institute of Technology, Huaian, Jiangsu 223001, China )

Received:2008-01-30 Revised:2008-05-13 Online:2008-06-25 Published:2008-06-25
Contact: CHENG Zhi-hui

Abstract

Abstract: A test of crude toxin toxicity using garlic root inhibition was examined from toxin production related to culture medium, temperature, pH, culturing duration, light, dark or shaking conditions. Investigations concerned a garlic white rot pathogen, Sclerotium cepivorum Berk. Results showed that optimum conditions included using a Fries liquid medium at pH 5 and at18 oC, with successive dark periods and shaking during culturing. Production of crude toxin reached the highest level on the 6th day of culturing.

Key words: garlic, Sclerotium cepivorum Berk., culturing conditions, crude toxin

CLC Number:

ZHANG lin-qing; and CHENG Zhi-hui. Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk. of Garlic[J]. ACTA HORTICULTURAE SINICA, 2008, 35(6): 841-846.

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Optimization of Culturing Conditions for Toxin Production by Sclerotium cepivorum Berk. of Garlic

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