Abstract: Due to its resistant characters to cold, insect and disease, Vitis amurensis Rupr. is one of the most important germplasm resource in grape breeding. It has male, female and hermaphrodite phenotype, but only hermaphrodite is suitable for commercial production. AFLP（amplifled fragment length polymorphism) was applied to identify markers associated with Vitis amurensis Rupr. sex type. By application of 64 markers in the screening, E-AGC/M-CTG was found to amplify a specific 283bp fragment in male and hermaphrodite pool. According to its sequencing result, SCAR (Sequence Characterized Amplified Region) primers were designed, and the primers gave the expected band in 97.6% of the tested 85 cultivars of Vitis amurensis Rupr.. It suggests that the SCAR marker will be useful in the seedling stage marker-assistant selection for breeding with the resistant Vitis amurensis Rupr.

Key words: Vitis amurensis Rupr., AFLP, SCAR, Molecular marker