Abstract: A F₂ population was derived from a cross between a heat sensitive line 01137 with a heat tolerance line CLN2001A of tomato. The colony was analyzed using SSR and RAPD techniques to select molecular markers related to tomato heat tolerance. One SSR marker and one RAPD marker were detected, and the genetic contribution rate of these markers for heat tolerance is 5.2% and 7.7% respectively. Two QTLs were mapped in linkage group 3 and linkage group 7. The SSR and RAPD techniques were used to evaluate the genetic diversity of 43 tomato germplasm materials about heat resistance and susceptibility. we use two markers（Tom59-60 and OPN02-600）which were significantly interrelated to the heat-tolerance of tomato to group the 43 tomato germplasm materials under two groups, the heat-tolerance and the heat-sensitive, and the veracity of grouping were 93.3% and 96.5% respectively.

Key words: tomato, heat tolerance, quantitative trait loci（QTL）