Isolation and Expression Analysis of Kunitz-type Enzyme Inhibitor GeneFragment in Potato (Solanum tube rosum L. )

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 403-406.

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Isolation and Expression Analysis of Kunitz-type Enzyme Inhibitor GeneFragment in Potato (Solanum tube rosum L. )

LI Guang-cun^1,2,JIN Li-ping¹,XIE Kai-yun¹,BIAN Chun-song¹,DUAN Shao-guang¹, and QU Dong-yu^1*

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²High-Tech Research Center, Shandong Academ y of Agricultural Sciences, Jipnan 250100, China)

Received:2006-07-20 Revised:2007-01-29 Online:2007-04-25 Published:2007-04-25
Contact: QU Dong-yu1

Abstract

Abstract:

The differentially exp ressed fragment, designated as kunitz2type enzyme inhibitor like (KTI-like) EST, was isolated from ED13 with high resistance to Ra lstonia solanacearum (Rs) using cDNA-RGA (resistance gene analogs, RGA). Results of BLAST against NCB I database showed that the KTI-like EST
shared a high similaritywith kunitz2type inhibitor genes, and might be one of the importantmembers of potato cysteine proteinase inhibitor (PCPI) gene family. Analysis of semi2quantity RT2PCR indicated that the KTI-like gene was induced by Rs and itwas trigged to express before 12 h and reached the highest level at about 48 h post-inoculation, then leveled off. Results of this study combined with the fact that PCPI is involved in plant defence against insects and pathogens indicated that the KTI2like gene might play an important role in plant resistance against Rs.

Key words: Potato, Potato bacterial wilt, cDNA-RGA, KTI-like EST, RT-PCR

CLC Number:

S 532

LI Guang-cun;JIN Li-ping;XIE Kai-yun;BIAN Chun-song;DUAN Shao-guang;and QU Dong-yu. Isolation and Expression Analysis of Kunitz-type Enzyme Inhibitor GeneFragment in Potato (Solanum tube rosum L. )[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 403-406.

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Isolation and Expression Analysis of Kunitz-type Enzyme Inhibitor GeneFragment in Potato (Solanum tube rosum L. )

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