Abstract: Based on the conserved sequence of the chitinase of crucifers, about 1 kb DNA fragment and
5′2 end fragment (150 bp) were amp lified by PCR from genomic DNA of Chinese cabbage. Experimentswere
performed on 72day2old Chinese cabbage seedlings after germination with salicylic acid of different concentra
tions. Total RNA was p repared from the p lantwith higher chitinase activity, and 3′2 end fragmentwas amp li
fied by 3′RACE. Comparing Chinese cabbage chitinase sequence cloned in this experimentwith that reported
in GenBank composed of 1 094 bp nucleotide of Brassica napus, the experiment showed that the chitinase se
quence of Chinese cabbage is 1 517 bp in size with one intron of 508 bp (DDBJ accession number:
AB257452) , and shares as high as 99% identitywith B. napus and over 50% with other high p lants in cod
ing region.

Key words: Chinese cabbage, Chitinase gene, Clone, RACE