cDNA Cloning and Expression of CaHSP26 from Sweet Pepper

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (6): 1247-1252.

• 研究论文 • Previous Articles Next Articles

cDNA Cloning and Expression of CaHSP26 from Sweet Pepper

Guo Shangjing^{1, 3};Guo Peng¹;Dong Xinchun¹;Meng Qingwei^{1, 2}

(¹College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China; ² Key Laboratory of Crop Biology of Shandong Province, Tai'an, Shandong 271018, China; ³College of Life Sciences, Liaocheng University, Liaocheng, Shandong 252000, China)

Received:2006-04-29 Revised:2006-07-02 Online:2006-12-25 Published:2006-12-25
Contact: Meng Qingwei

Abstract

Abstract: Full length 917 bp of chloroplast sHSP cDNA was isolated from heat-shocked sweet pepper leaves by RT-PCR approach, named CaHSP26 (AY224603 ). A single-gene was found in sweet pepper genomic DNA by southern-blot analysis. Northern blot revealed that heat stress induced the expression of CaHSP26 in roots, stems and leaves of sweet pepper. The heterologous expression of CaHSP26 in E. coli could maintain higher cell viability of E. coli under high temperature stress ( 50℃). These results indicated that plant chloroplast sHSP could p rotect cell against heat stress.

Key words: Sweet pepper, Small heat shock protein (sHSP), Gene cloning, Heterologous expression

CLC Number:

S641.3

Guo Shangjing;;Guo Peng;Dong Xinchun;Meng Qingwei;. cDNA Cloning and Expression of CaHSP26 from Sweet Pepper[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1247-1252.

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cDNA Cloning and Expression of CaHSP26 from Sweet Pepper

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