Embryogenesis and Doubling Mechanism of Isolated Microspore Culture inChinese Cabbage ( Brassica rapa ssp. pekinensis)

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 974-978.

• 研究论文 • Previous Articles Next Articles

Embryogenesis and Doubling Mechanism of Isolated Microspore Culture inChinese Cabbage ( Brassica rapa ssp. pekinensis)

Li Fei;Zhang Shujiang;Zhang Shifan;Niu Xinke;Sun Rifei

( Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:2006-01-06 Revised:2006-04-27 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: The pathway of nuclear divisions of isolated microspore at different development stages and their embryogenesis p rocess in Chinese cabbage were investigated under a fluorescence microscopy by using DAPI staining to understand embryogenesis and doublingmechanism of isolated microspore culture in Chinese cabbage. The results showed that after heat shock pretreatment the isolated microspores expanded. Most of microspores exhibited B pathway, and nuclear chromosomes doubled at very early stage, then doubled haploid embryoids formed. That iswhy isolated microspore culture of Chinese cabbage usually p roduce high frequency of spontaneously doubled haploid plants.

Key words: Chinese cabbage, Microspore culture, DAPI fluorescence stain, Embryogenesis, Spontaneously doubled haploid

Li Fei;Zhang Shujiang;Zhang Shifan;Niu Xinke;Sun Rifei. Embryogenesis and Doubling Mechanism of Isolated Microspore Culture inChinese Cabbage ( Brassica rapa ssp. pekinensis)[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 974-978.

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Embryogenesis and Doubling Mechanism of Isolated Microspore Culture inChinese Cabbage ( Brassica rapa ssp. pekinensis)

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