Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (5): 1093-1095.

• 研究报告 • Previous Articles Next Articles

Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages

Yu Zhandong^{1, 2};Zhao Shuangyi³;He Qiwei^1*;Wang Cuihua¹;Mou Jinhua¹; Liu Chunxiang¹;Kang Jing²

(¹ Institute of Vegetable Crops, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong 250100, China; ²College of Horticulture, Jilin Agricultural University, Changchun, Jilin 130118, China; ³College of Life Sciences, Shandong University, Ji'nan,Shandong 250100, China)

Received:2005-10-19 Revised:2006-01-23 Online:2006-10-25 Published:2006-10-25

Abstract

Abstract: Agrobacterium tumiefaciens-mediated regeneration system was established using Anti-TuMV-Nib gene. Cotyledons with petiole from aseptic seedlings of Chinese cabbage (Brassica campestris ssp. pekinensis‘Fushanbaotou’) were used as explants and the transgenic plants were obtained. The analysis of PCR-Southern blotting, RT-PCR and ELISA detection showed that the target gene was integrated into the Chinese cabbage genome and expressed.

Key words: Chinese cabbage, TuMV, Nib gene, Genetic transformation

Yu Zhandong;;Zhao Shuangyi;He Qiwei;Wang Cuihua;Mou Jinhua;Liu Chunxiang;Kang Jing. Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages[J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1093-1095.

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Genetic Transformation of Anti-TuMV-Nib into Transgenic Chinese Cabbages

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