Determination of Monomeric and Polymeric Flavan-3-ols in Grape Berry byHigh Performance Liquid Chromatography

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 714-720.

• 研究论文 • Previous Articles Next Articles

Determination of Monomeric and Polymeric Flavan-3-ols in Grape Berry byHigh Performance Liquid Chromatography

Wen Pengfei^{1, 2};Chen Jianye¹;Li Jingming¹;Wan Sibao¹;Kong Weifu¹; HuangWeidong^1*

( ¹College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; ²College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China)

Received:2005-10-18 Revised:2006-01-04 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: The grape (Vitis vinifera L. ‘Cabernet Sauvignon’) berry was used as the material. A new method was developed for simultaneously determinating five kinds ofmonomeric flavan-3-ols and their derivatives, including ( + ) -catechin (CAT) , ( - ) -epigallocatechin ( EGC) , ( - ) -epigallocatechin gallate ( EGCG) , ( - ) -epicatechin ( EC) , and ( - )-epicatechin gallate ( ECG) , in the grape berry by high performance liquid chromatography (HPLC). These compounds were efficiently separated directly from the samples through a chromatographic column of 5 μm LiChrospher 100 RP-18e (250 mm ×410mm I.D., Merck)
by the mobile phase gradient elution of glacial acetic acid solution at the flow rate of 1.0 mL /min, at the detection wavelength of 280 nm and at 30℃. The polymeric flavan-3-ols had been fractioned and purified on TSK HW-50 ( F) , followed by acid-catalyzed degradation in the presence of phloroglucinol, then the mean degree and the composition of polymeric flavan-3-ols in grape berry were determined by HPLC. The results showed that monomeric and polymeric flavan-3-ols were mainly accumulated in the skins and seeds of grape berry.

Key words: Grape, Berry, HPLC, Flavan-3-ols, Polymeric flavan-3-ols

Wen Pengfei;;Chen Jianye;Li Jingming;Wan Sibao;Kong Weifu;HuangWeidong. Determination of Monomeric and Polymeric Flavan-3-ols in Grape Berry byHigh Performance Liquid Chromatography[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 714-720.

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Determination of Monomeric and Polymeric Flavan-3-ols in Grape Berry byHigh Performance Liquid Chromatography

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