Ultrastructural Changes Associated with Droplet Vitrification Cryopreservation of Banana Shoot-tip

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (4): 709-713.

• 研究论文 • Previous Articles Next Articles

Ultrastructural Changes Associated with Droplet Vitrification Cryopreservation of Banana Shoot-tip

Zhang Shoumei¹;Li Jianguo^{1, 2*};Chen Houbin¹;Xu Chunxiang¹;Han Lina¹

(¹ Tropical and Subtropical Laboratory, South China Agricultural University, Guangzhou, Guangdong 510642, China; ²Institute of Biotechnology on Horticultural Crop, South China Agricultural University, Guangzhou, Guangdong 510640, China)

Received:2005-11-09 Revised:2006-02-14 Online:2006-08-25 Published:2006-08-25

Abstract

Abstract: Ultrastructure of banana shoot-tip cells during drop let vitrification cryopreservation was observed by using the transmission electron microscopy ( TEM). The results showed that loading decreased the degree of vacuolation and caused plasmolysis. The extent of plasmolysis became more severe and protoplast was condensed after dehydrating treatment. Meanwhile, cell walls, cellmembranes and nucleus envelopes of some cellswere lethally injured. Although there were some changes in the structure of many other cells, the
damage was reversible, which might be rehabilitated automatically during recovery culture and regenerating plantlets could be obtained. Cells were mainly damaged during the dehydrating process, and no significant alterations occurred subsequently in the cooling step. The possible mechanisms for those resultswere discussed.

Key words: Banana, Cryopreservation, Droplet vitrification, Ultrastructrue

Zhang Shoumei;Li Jianguo;;Chen Houbin;Xu Chunxiang;Han Lina. Ultrastructural Changes Associated with Droplet Vitrification Cryopreservation of Banana Shoot-tip[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 709-713.

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Ultrastructural Changes Associated with Droplet Vitrification Cryopreservation of Banana Shoot-tip

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