Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (5): 633-636.

• 研究论文 • Previous Articles Next Articles

Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene

Yu Yixun;Bao Manzhu^*

( Key Laboratory of Horticultural Plant Biology , Ministry of Education , College of Horticulture and Forestry Sciences ,Huaz hong Agricultural University , Wuhan 430070 ,China)

Received:2003-10-25 Revised:2004-02-18 Online:2004-10-25 Published:2004-10-25
Contact: Bao Manzhu

Abstract

Abstract: Taking young leaf as explants , the carnation ( Dianthus caryophyllus L. ) cultivar‘Yellow Star’was transformed with antisense genomic DNA of ACC oxidase , the key enzyme in ethylene synthesis ,driven by the CaMV 35S promoter mediated by A grobacterium tumefacien. PCR analysis and Southern blotting detection showed that foreign gene was integrated into the carnation genome. It is demonst rated that both leaf age and the acetosyringone are effective factors that influences the differentiation fequency on selection medium. The chimera occurring rate was effectively decreased by two times regeneration of the leaf explants under selection. The transgenic plants were t ransplanted to soil and flowering normally in isolated greenhouse. The vase life of t ransgenic plant is 4 days longer than that of cont rol plants under 25 ℃.

Key words: Carnation, ACC oxidase (ACO) gene, Genetic transformation, Vase life

CLC Number:

S 68

Yu Yixun;Bao Manzhu. Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene[J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 633-636.

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Carnation Flower Vase Life Prolonged by Transformation with Antisense ACCOxidase Gene

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