Gene Expression Associated 、 th Dominant M ale Sterility in Cabbage isRevealed by cDNA-Am

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (6): 668-672.

• 研究论文 • Previous Articles Next Articles

Gene Expression Associated 、 th Dominant M ale Sterility in Cabbage isRevealed by cDNA-Am

Lou Ping ，Wang Xiaowu ，Guusje．Bonnema2，and Fang Zhiyua

( Institute ofVegetables and Flowers， Chinese Academy ofAgricuhural Sciences，8e0

Received:2002-12-06 Revised:2003-03-05 Online:2003-12-25 Published:2003-12-25

Abstract

Abstract: Abstract： cDNA—AFLP was used to detect diferentially expressed genes during flower bud development of
male sterile an d fertile lines of cabbage an d broccoli． Expression profiles were generated around 26，000 cDNA frag—
ments(128 primer combinations)．Transcriptional changes were observed for 24 cDNA fragments，from which，
1 3 were only expressed in fertile buds， while the other 1 1 were upregulated in fertile buds． Sequencing of a 300 bp
diferential fragment amplified by primer combination A16T15 revealed 89％ similarity to the gene thioredoxin，
which is related to pollen development in the Brassica family．

Key words: Cabbage, Dominant male sterility, cDNA—AFLP, Diferentially expressed genes

CLC Number:

s 635

Lou Ping;Wang Xiaowu;Guusje．Bonnema;and Fang Zhiyua. Gene Expression Associated 、 th Dominant M ale Sterility in Cabbage isRevealed by cDNA-Am[J]. ACTA HORTICULTURAE SINICA, 2003, 30(6): 668-672.

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Gene Expression Associated 、 th Dominant M ale Sterility in Cabbage isRevealed by cDNA-Am

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