The Establishm ent of M ultiplex ——CAPS Technique and Its Application for Genetic Characterization in Tomato

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (6): 673-677.

• 研究论文 • Previous Articles Next Articles

The Establishm ent of M ultiplex ——CAPS Technique and Its Application for Genetic Characterization in Tomato

W ang Xiaoxuan ， Du Yongchen ， Zhu Dewei ， Luigi Monti2
，S． Grandillo

(‘Institute ofVegetables and Flowers，Chinese Academ)ofAgricultural Science，Beijing 100081，China； CNR-IMOF，Research In—stitulefor Vegetable and Ornamental Plant Breeding，Portici 80055，haly)

Received:2003-02-24 Revised:2003-07-25 Online:2003-12-25 Published:2003-12-25

Abstract

Abstract: An eficient an d reliable technique Multiplex— CAPS was developed for genetic characterization by
combination of two principles Multi—PCR and CAPS (Cleaved amplified polymorphic sequence) in tomato．This
techniqu e included (1)using 2 or 3 or more than 3 primer pairs in the same am plification reaction，after 30—40
thermal cycles the PCR yield diferent products which correspond to each individual primer pair， (2) using only
one enz、111e to digest these diferent PCR products， (3)Using agrose gel electrophoresis to detect polymorphisms
followed by ethidium staining． By comparison with CAPS， Multiplex— CAPS Can detect the polymorphism of 2 or 3
or more than 3 primer pairs in the same am plification reaction at the same time． Th e polymorphism of Multiplex—
CAPS equal exactly the polymorphism supe rimpose of each individual primer pair． Multiplex— CAPS has good re—
producibility with high an alytical efect， lOW cost， lOW labor an d time consuming．

Key words: Tomato, Multiplex— CAPS, CAPS, Multiplex—PCR

CLC Number:

S 641．2

W ang Xiaoxuan;Du Yongchen;Zhu Dewei;Luigi Monti;S． Grandillo. The Establishm ent of M ultiplex ——CAPS Technique and Its Application for Genetic Characterization in Tomato[J]. ACTA HORTICULTURAE SINICA, 2003, 30(6): 673-677.

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The Establishm ent of M ultiplex ——CAPS Technique and Its Application for Genetic Characterization in Tomato

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