Identification of RAPD and SCAR Markers Linked to the Single2double FlowerGene in Japanese Apricot ( Prunus mume Sieb. et Zucc. )

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (5): 612-614.

• 研究报告 • Previous Articles Next Articles

Identification of RAPD and SCAR Markers Linked to the Single2double FlowerGene in Japanese Apricot ( Prunus mume Sieb. et Zucc. )

Gao Zhihong^1,2;Han Zhenhai¹;Zhang Zhen²;Zhang Yuming²

(¹Institute for Horticultural Plant,China Agricultural University,Beijing 100094 , China ; ²College of Horticulture , Nanjing Agricultural University , Nanjing 210095 , China)

Received:2002-11-29 Revised:2003-02-21 Online:2003-10-25 Published:2003-10-25
Contact: Zhang Zhen

Abstract

Abstract: Randomly amplified polymorphic DNA ( RAPD) and sequence characterized amplified region (SCAR) marker were performed to detect markers linked to the single double flower in Japanese apricot ( Prunus mume Sieb. et Zucc. ) . Primer OPP01 was identified from 34 10-mer primers examined. The fragment was cloned and sequenced as 1899 base pairs. According to the sequence , one primer pairs were designed to convert the RAPD marker to SCAR marker. The length of the amplified fragment of SCAR marker was 1079 base pairs.

Key words: Japanese apricot, Double flower, Single flower, Molecular marker

CLC Number:

S 685.17

Gao Zhihong;Han Zhenhai;Zhang Zhen;Zhang Yuming. Identification of RAPD and SCAR Markers Linked to the Single2double FlowerGene in Japanese Apricot ( Prunus mume Sieb. et Zucc. )[J]. ACTA HORTICULTURAE SINICA, 2003, 30(5): 612-614.

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Identification of RAPD and SCAR Markers Linked to the Single2double FlowerGene in Japanese Apricot ( Prunus mume Sieb. et Zucc. )

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