https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (5): 589-591.

• 研究报告 • Previous Articles     Next Articles

Cloning of Tomato myb Gene Fragments and Expression upon Development ofHypersensitive Response

Cai Xinzhong1;Xu Youping2;Lou Jian2   

  1. (1 Department of Plant Protection , Zhejiang University , Hangzhou 310029 , China ;2 Center of Analysis and Measurement , Zhejiang University , Hangzhou 310029 , China)
  • Received:2002-09-09 Revised:2003-01-06 Online:2003-10-25 Published:2003-10-25

Abstract: Two 215 bp myb gene fragments,LeMYB1 and LeMYB2 with GenBank accession No. AY131230 and AY131231 respectively , were cloned through RT - PCR. The primers were designed in accordance with tobacco myb1 at the DNA-binding domain conserved in MYB , and the template was the RNA isolated from tomato seedlings showing hypersensitive responses (HR) . Only nucleotide 63 was different between the two fragments , of which LeMYB1 was A and LeMYB2 was G. Three nucleotides within the primers for cloning of LeMYB1 were different between LeMYB1 and counterpart of THM18,whereas 90.7% nucleotide sequence of LeMYB1 was identical to corresponding parts of tobacco myb1. Northern assay revealed that expression of LeMYB1-related genes were
induced upon occurrence of hypersensitive necrosis in tomato seedlings and enhanced henceforth. Their coincident expression with HR development in tomato indicated that LeMYB1-related genes might be involved in regulation of
HR and disease resistance conferred by tomato fungal resistance gene Cf-9.

Key words: Tomato, LeMYB, Transcription factor, Gene expression, Cf-9, Hypersensitive response

CLC Number: