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ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (5): 438-442.

• 研究论文 • Previous Articles     Next Articles

Identification of Double Triplo-3, 6 and Acquisition of Primary Triplo-3 andTriplo-6 in Chinese Cabbage

Shen Shuxing1, Li Zhenqiu2, Zhang Chenghe1, Wang Yanhua1, and Chen Xueping1   

  1. (1 Departerment of Horticulture , Hebei Agricultural University , Baoding 071001 , China ; 2 College of Life Science , Hebei University , Baoding 071001 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-10-25 Published:2002-10-25

Abstract: An autotetraploid Chinese cabbage 〔Brassica campestris ssp. pekinensis (Rupr.) Olsson〕microspore line 940521 was designated as double triplo23 , 6 by mitotic chromosome karyotype analysis. By meiosis metaphase Ⅰchromosome observation , triplo23 , triplo26 and double triplo23 , 6 were identified in the F1 of line 940521 ×Guan 291. The transmission of extra chromosomes in double triplo23 , 6 was calculated and the result
showed that the rates of No. 3 and No. 6 chromosome were 28. 26 % and 47. 83 % respectively. The pollens of triplo23 , triplo26 and double triplo23 , 6 were stained with TTC and their activities were measured. The result
showed that the differences of pollen activity among triplo23 , triplo26 and double triplo23 , 6 were significant at 0. 01 level .

Key words: Chinese cabbage, Chromosome, Primary trisomic, Double trisomics

CLC Number: